Acetone extract from Streptoverticillium sp., a bacterium isolated from Brazilian cerrado soil, induces anti-infl ammatory activity in mice

Abstract

The Streptoverticillium sp. Z1 is an actinomycete isolated from the soil under Cerrado vegetation, the extract of this strain was investigated in nociceptive and infl ammatory models. The Streptoverticillium extract (ExS) 50 and 100 mg/kg (s.c.) produced a signifi cant inhibition of acetic acid-induced abdominal writhings thereby demonstrating an anti-nociceptive effect. In the tail fl ick test the ExS (s.c.) was inactive. This result implited that ExS does not contain opioid-like compounds with central analgesic properties. In the infl ammatory models, ExS 100 and 200 mg/kg (s.c.) were able to inhibit the croton oil-induced ear edema and, ExS 200 and 500 mg/kg (s.c.) inhibited the leukocyte migration on the carrageenan-induced peritonitis. The phospholipase A 2 enzymatic assay showed that the anti-infl ammatory activity of ExS was not due to direct effect on phospholipase A 2 activity. These data suggest that Streptoverticillium sp. produces metabolites with anti- infl ammatory effect and that these metabolites are unable to directly inhibit phospholipase A 2 enzyme.