An EPR spin probe study of the interactions between PC liposomes and stratum corneum membranes

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dc.creatorMendanha Neto, Sebastião Antônio
dc.creatorAnjos, Jorge Luiz Vieira dos
dc.creatorSilva, Lorena Maione
dc.creatorSilva, Halanna Cristina Barbosa
dc.creatorLima, Eliana Martins
dc.creatorAlonso, Antonio
dc.date.accessioned2023-05-30T14:56:33Z
dc.date.available2023-05-30T14:56:33Z
dc.date.issued2018
dc.description.abstractThe electron paramagnetic resonance (EPR) spin labeling methodology was used to analyze the interactions of phosphatidylcholine (PC) liposomal formulations that are commonly used as transepidermal drug delivery systems with stratum corneum (SC) membranes. The lipid dynamics of five liposome formulations were eval uated to study the influences of sorbitan monooleate (Span80), cholesterol, and cholesterol with the charged lipids 2-dioleoyl-3-trimethylammonium-propane (DOTAP) and 1,2-distearoyl-sn-glycero-3-phospho-(1′-rac-gly cerol) (DSPG) on the molecular dynamics of PC vesicles. The EPR spectra of 5-doxyl-stearic acid (5-DSA) showed that the addition of Span80 to the liposomes increased the lipid fluidity, whereas cholesterol had the opposite effect, and the combination of charged lipids and cholesterol did not additionally influence the lipid bilayer dynamics. Fatty acid spin-labeled SC membranes were treated with the liposome formulations, leading to mi gration of the spin label to the molecular environment of the formulation and the presence of two spectral components representing distinct mobility states. In terms of molecular dynamics, these environments corre spond to the lipid domains of the untreated SC and the liposome, indicating a poor interaction between the liposome and SC membranes. However, the contact was sufficient to allow a pronounced exchange of the spin labeled fatty acid. Our data suggest that flexible liposomes may access the inner intercellular membranes of the SC and facilitate mutual lipid exchange without losing their relative liposomal integrity.pt_BR
dc.identifier.citationMENDANHA, Sebastião Antonio; ANJOS, Jorge Luiz Veira dos; MAIONE-SILVA, Lorena; SILVA, Halanna Cristina B.; LIMA, Eliana Martins; ALONSO, Antonio. An EPR spin probe study of the interactions between PC liposomes and stratum corneum membranes. International Journal of Pharmaceutics, Amsterdam, v. 545, p. 93-100, 2018. DOI: 10.1016/j.ijpharm.2018.04.057. Disponível em: https://www.sciencedirect.com/science/article/pii/S0378517318302850?via%3Dihub. Acesso em: 26 maio 2023.pt_BR
dc.identifier.doi10.1016/j.ijpharm.2018.04.057
dc.identifier.issn0378-5173
dc.identifier.issne- 1873-3476
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/22632
dc.language.isoengpt_BR
dc.publisher.countryHolandapt_BR
dc.publisher.departmentInstituto de Física - IF (RMG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectFlexible liposomespt_BR
dc.subjectSpin labelspt_BR
dc.subjectEPRpt_BR
dc.subjectTransepidermal drug deliverypt_BR
dc.titleAn EPR spin probe study of the interactions between PC liposomes and stratum corneum membranespt_BR
dc.typeArtigopt_BR

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