Excretion of Brucella abortus vaccine B19 strain during a reproductive cycle in dairy cows

dc.creatorPacheco, Wanessa Andrade
dc.creatorGenovez, Margareth Elide
dc.creatorPozzi, Cláudia Rodrigues
dc.creatorSilva, L. M. P.
dc.creatorAzevedo, Sergio Santos de
dc.creatorDid, C. C.
dc.creatorPiatti, Rosa Maria
dc.creatorPinheiro, Eliana Scarcelli
dc.creatorCastro, Vanessa
dc.creatorMiyashiro, Simone
dc.creatorGambarini, Maria Lúcia
dc.description.abstractThis paper aimed to determine the excretion period of B19 vaccine strain during a complete reproductive cycle (from estrus synchronization, artificial insemination, pregnancy and until 30 days after parturition) of dairy cows from 3 to 9 years old that were previously vaccinated from 3 to 8 months. Three groups were monitored with monthly milk and urine collection during 12 months: G1 with seven cows from 3 to 4 years old; G2 with three cows from 5 to 6 years old; and G3 with four cows from 7 to 9 years old. Urine and milk samples were submitted to bacteriological culture and urine and PCR reactions for detection of Brucella spp. and PCR–multiplex for B19 strain identification. Ring test (RT) was also performed in the milk samples, and serum samples were tested by buffered acidified plate antigen test (BAPA). All animals were serologically negative at BAPA and Brucella spp. was not isolated from both urine and milk samples. RT revealed 13/210 (6.2%) positive milk samples. PCR reactions detected DNA of Brucella spp. in 86/420 (20.5%) samples. In urine it was found a significantly higher frequency (35.2%; 74/210) than in milk (5.7%; 12/210), more frequently from the estrus to 150 days of pregnancy and after parturition (6.7%; 10/150), and from 150 days of pregnancy to parturition (3.4%; 2/60), and they were all identified as B19 strain. In three groups, intermittent excretion of B19 strain was detected mainly in urine samples, which confirmed its multiplication and persistence in cows for until 9 years.pt_BR
dc.identifier.citationPACHECO, W. A.; GENOVEZ, M. E.; POZZI, C. R.; SILVA, L. M. P.; AZEVEDO, S. S.; DID, C. C.; PIATTI, R. M.; PINHEIRO, E. S.; CASTRO, V.; MIYASHIRO, S.; GAMBARINI, M. L. Excretion of Brucella abortus vaccine B19 strain during a reproductive cycle in dairy cows. Brazilian Journal of Microbiology, São Paulo, v. 43, n. 2, p. 594-601, Apr./June 2012.pt_BR
dc.identifier.issne- 1678-4405
dc.publisher.departmentEscola de Veterinária e Zootecnia - EVZ (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectBovine brucellosispt_BR
dc.subjectB19 vaccinept_BR
dc.titleExcretion of Brucella abortus vaccine B19 strain during a reproductive cycle in dairy cowspt_BR


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