Electrochemical behavior of crude extract of Brosimum gaudchaudii and its major bioactives, psoralen and bergapten
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2016-10-10
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Roots from Brosimum gaudchaudii Trécul (Moraceae), popularly known as Mama-Cadela, is a
traditional medicinal shrub from Brazilian “Cerrado” widely used in the photochemotherapy of
vitiligo. Their main actives are the prenylated coumarins, Psoralen (Ps) and bergapten (Bp), which
under UV exposure generate active compounds that form adducts with DNA. Though such reactions
may involve electron transfer reactions, the redox behavior of BGT and their major isolated
furanocoumarins, remain unknown. Therefore the aim of this study was to evaluate the electrochemical
behavior of Ps, Bp and crude extracts of BGT by means of voltammetry. The cyclic voltammograms
performed for Bp and Ps, confirmed the electroactivity only for Bp, which presented a anodic peak
potential at Ep1a = 1.2 V. Similar behavior was observed for the BGT crude extract. In turn, from the
differential pulse voltammograms (DPV), it was observed two small anodic peaks, 1a and 2a at lower
peak potentials, which are consistent with hydroquinone and catechol like compounds. Also, it was
observed that the main anodic peak occurred close to the Bp peak potential, 1a, whereas no peak was
observed for Ps. Therefore, from voltammetric assays, it could be inferred that Bp is present in BGT
crude extracts, which were corroborated by HPLC coupled to mass spectrometry. An cheaper and
simpler electroanalytical procedure was proposed in order to estimate the amount of Bp in BGT, which
despite the low repeatability presented comparable recovery compared to HPLC-PDA.
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Bergapten, Psoralen, Vitiligo, Psoriasis, Mama-cadela
Citação
ALECRIM, M. F. et al. Electrochemical behavior of crude extract of Brosimum gaudchaudii and its major bioactives, psoralen and bergapten. International Journal of Electrochemical Science, Amsterdam, v. 11, p. 9519-9528, 2016. DOI: 10.20964/2016.11.31. Disponível em: http://www.electrochemsci.org/papers/vol11/111109519.pdf. Acesso em: 21 jun. 2023.