1H HR-MAS NMR and S180 cells: metabolite assignment and evaluation of pulse sequence
Data
2014-04
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Resumo
High resolution magic angle spinning 1H nuclear magnetic resonance spectroscopy (HR-MAS
NMR) is a useful technique for evaluation of intact cells and tissues. However, optimal NMR
parameters are crucial in obtaining reliable results. To identify the key steps for the optimization
of HR-MAS NMR parameters, we assessed different pulse sequences and NMR parameters using
sarcoma 180 (S180) cells. A complete assignment of the metabolites of S180 is given to assist
future studies.
Descrição
Palavras-chave
HR-MAS NMR, Sarcoma 180, S180, CPMG
Citação
OLIVEIRA, Aline L. de et al. 1H HR-MAS NMR and S180 cells: metabolite assignment and evaluation of pulse sequence. Journal of the Brazilian Chemical Society, Campinas, v. 25, n. 6, p. 1135-1142, Apr. 2014