Tetracarboxy-phthalocyanines: from excited state dynamics to photodynamic inactivation against Bovine herpesvirus type 1

Cocca, Leandro Henrique Zucolotto; Oliveira, Taise Maria dos Anjos; Gotardo, Fernando; Teles, Amanda Vargas; Menegatti, Ricardo; Siqueira, Jonathas de Paula; Mendonça, Cleber Renato; Bataus, Luiz Artur Mendes; Ribeiro, Anderson Orzari; Souza,  Thalita Ferrreira Menegassi de; Gonçalves, Pablo José

Tetracarboxy-phthalocyanines: from excited state dynamics to photodynamic inactivation against Bovine herpesvirus type 1

Data

2017

Autores

Cocca, Leandro Henrique Zucolotto

Oliveira, Taise Maria dos Anjos

Gotardo, Fernando

Teles, Amanda Vargas

Menegatti, Ricardo

Siqueira, Jonathas de Paula

Mendonça, Cleber Renato

Bataus, Luiz Artur Mendes

Ribeiro, Anderson Orzari

Souza, Thalita Ferrreira Menegassi de

Resumo

Herein we present the excited state dynamic of zinc and aluminum tetracarboxy-phthalocyanines (ZnPc and AlPc) and its application in the photodynamic inactivation (PDI) of Bovine herpesvirus type 1 (BoHV-1) in vitro. The excited state dynamic provides valuable data to describe the excited state properties of potential optical limiters and/or photosensitizers (PSs), such as: the excited state cross-sections, fluorescence lifetime and triplet state quantum yield. The excited state characterization was performed using three different Z-scan techniques: Single Pulse, White Light Continuum and Pulse Train. Considering the photodynamic inactivation of BoHV-1, an initial viral suspension containing 105.75 TCID50/mL was incubated with the PSs for 1 h at 37 °C under agitation and protected from light. The samples were placed in microtiter plates and irradiated (180 mW/cm2). During irradiation, a sample was taken every 15 min and the viability of the virus was evaluated. The results show that both phthalocyanines were efficient against viruses. However, a higher photodynamic efficiency was observed by ZnPc, which can be attributed to its higher triplet and singlet quantum yields. The results presented here are important for animal health (treatment of BoHV-1) and also open up a field of studies to use AlPc and ZnPc as potential agents against a wide range of microorganisms of veterinary interest.

Citação

COCCA, Leandro H. Z. et al. Tetracarboxy-phthalocyanines: from excited state dynamics to photodynamic inactivation against Bovine herpesvirus type 1. Journal of Photochemistry and Photobiology B: biology, Amsterdam, v. 175, p. 1-8, 2017. DOI: 10.1016/j.jphotobiol.2017.08.019. Disponível em: https://www.sciencedirect.com/science/article/pii/S1011134417304694. Acesso em: 3 jul. 2024.