Immunoblotting analyses using two-dimensional gel electrophoresis of Trypanosoma cruzi excreted-secreted antigens
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Data
2004-12
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Resumo
Trypanosoma cruzi trypomastigotes excrete-secrete a complex mixture of antigenic molecules. This antigenic mixture
denominated trypomastigote excreted-secreted antigens contains a 150-160 kDa band that shows excellent performance
in Chagas’ disease diagnosis by immunoblotting. The present study partially characterized by two-dimensional gel
electrophoresis the immunoreactivity against the 150-160kDa protein using sera samples from chagasic patients in different
phases of the disease. Trypomastigote excreted-secreted antigen preparations were subjected to high-resolution two-
dimensional (2D) gel electrophoresis followed by immunoblotting with sera from chagasic and non-chagasic patients. The
150-160kDa protein presented four isoforms with isoelectric focusing ranging from 6.2 to 6.7. The four isoforms were
recognized by IgM from acute phase and IgG from chronic phase sera of chagasic patients. The 150-160kDa isoform with IF
of approximately 6.4 became the immunodominant spot with the progression of the disease. No cross-reactivity was observed
with non-chagasic or patients infected with Leishmania sp. In this study we provide basic knowledge that supports the
validation of trypomastigote excreted-secreted antigens for serological diagnosis of Chagas’ disease.
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Trypanosoma cruzi, Immunoblotting, Excreted-secreted antigens, Two-dimensional gel electrophoresis
Citação
SILVA, Adriano Gomes; SILVEIRA-LACERDA, Elisangela Paula; CUNHA-JÚNIOR, Jair Pereira; SOUZA, Maria Aparecida de; FAVORETO JUNIOR, Sílvio. Immunoblotting analyses using two-dimensional gel electrophoresis of Trypanosoma cruzi excreted-secreted antigens. Revista da Sociedade Brasileira de Medicina Tropical, Uberaba, v. 37, n.6, p. 454-459, Nov./Dec. 2004.