Antimicrobial potential of ozone in ultrasonic cleaner system against Staphylococcus aureus

Resumo

The aim of this study was to evaluate the antimicrobial potential of ozone applied to 3 different solutions in an ultrasonic cleaning system against Staphylococcus aureus. A total of 120 mL of S. aureus were mixed in 6 L of the experimental solutions (sterilie distilled water, vinegar and sterile distilled water + Endozime AWpluz) used in a ultrasonic cleaning system (UCS). Ozone was produced by an electric discharge through a current of oxygen and bubbling with flow rate at 7g/h ozone (1.2%) into the microbial suspensions. Ten mL of each experimental suspension were collected and 5 fold dilutions were made in 9 mL of BHI and incubated at 37°C for 48 h. Bacterial growth was evaluated by turbidity of the culture medium. At the same time, 1 mL of bacterial samples was collected and inoculated in BHIA plates. After incubation at 37°C for 48 h, the number of colony forming units (cfu) per mL on BHIA surface was counted. In dilution test in BHI tubes and in BHIA plates (cfu/mL), bacterial growth was not observed in any of the experimental solutions when ozone was added. Under the tested conditions, it may be concluded that the addition of ozone to a ultrasonic cleaning system containing different experimental solutions resulted in antibacterial activity against S. aureus.

Descrição

Palavras-chave

Ozone, Dental treatment units, Ultrasonic cleaner system, Staphylococcus aureus, Water disinfection

Citação

ESTRELA, Carlos et al. Antimicrobial potential of ozone in ultrasonic cleaner system against staphylococcus aureus. Brazilian Dental Journal, Ribeirão Preto, v. 17, n. 2, p. 134-138, 2006.