Vascular relaxing effect of Hydrocotyle umbellata L. is mediated by blocking of l-type Ca2+ channels

Página do item simplificado
dc.creatorBufáiçal, Daniela Medeiros Lobo de Andrade
dc.creatorCorreia, Mikaelle Costa 
dc.creatorOliveira, Matheus Gabriel de
dc.creatorSantos, Eder Soares de Almeida
dc.creatorNeves, Bruno Junior
dc.creatorPaula, José Realino de
dc.creatorRocha, Matheus Lavorenti
dc.date.accessioned2024-09-12T14:25:41Z
dc.date.available2024-09-12T14:25:41Z
dc.date.issued2022
dc.description.abstractEthnopharmacological relevance Hydrocotyle umbellata L. is a medicinal herb for the treatment of some health problems including hypertension, according to traditional medicine. Even so, its vascular effects and the pharmacological action mechanisms have not been analyzed. Aim of the study This experiment aimed to analyze the effects of hydroalcoholic extract of Hydrocotyle umbellata L. (HEHU) on isolated vessels and verify the interaction of hibalactone (chemical marker) against Cav1.2 channels using molecular docking. Materials and methods Vascular reactivity experiments were performed using rat aortas with (E+) or without endothelium (E−) in an isolated organ bath. Computational molecular docking approaches were used to show the direct effect on L-type Ca2+ Channels. Results HEHU (0–560 μg/mL) induced relaxation of the pre-contracted arteries in a concentration-dependent manner. The maximum effect was higher in E+ (76.8 ± 4.1%) as compared to E− (47.3 ± 5.5%). Pre-treatment of E+ arteries with L-NAME or ODQ reduced the relaxation to similar level of E− arteries. The treatment of arteries with MDL-12,330 A, diclofenac, propranolol and atropine did not change the relaxation induced by HEHU. The contraction caused by internal Ca2+ release induced by caffeine was reduced after HEHU treatment. Moreover, the HEHU also impaired the contraction induced by Ca2+ influx stimulated with phenylephrine or high KCl. The docking study demonstrated the effectiveness of hibalactone in blocking the Cav1.2 channel. Conclusions These findings show that HEHU induces vascular relaxation which is potentiated (but not dependent) by endothelial cells. Blocking of Ca2+ influx seems to be the main mechanism for the vascular effects of HEHU.
dc.identifier.citationANDRADE, Daniela M. L. de et al. Vascular relaxing effect of Hydrocotyle umbellata L. is mediated by blocking of l-type Ca2+ channels. Journal of Ethnopharmacology, Amsterdam, v. 289, e115019, 2022. DOI: 10.1016/j.jep.2022.115019. Disponível em: https://www.sciencedirect.com/science/article/pii/S0378874122000538. Acesso em: 3 set. 2024.
dc.identifier.doi10.1016/j.jep.2022.115019
dc.identifier.issn0378-8741
dc.identifier.issne- 1872-7573
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0378874122000538
dc.language.isoeng
dc.publisher.countryHolanda
dc.publisher.departmentFaculdade de Farmácia - FF (RMG)
dc.rightsAcesso Restrito
dc.subjectHydrocotyle umbellata L.
dc.subjectVascular relaxation
dc.subjectEndothelium
dc.subjectCalcium channel
dc.subjectHibalactone
dc.titleVascular relaxing effect of Hydrocotyle umbellata L. is mediated by blocking of l-type Ca2+ channels
dc.typeArtigo

Arquivos

Licença do Pacote
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Baixar

Coleções

FF - Artigos publicados em periódicos