Analyses of the development and glycoproteins present in the ovarian follicles of Poecilia vivipara (Cyprinodontiformes, Poeciliidae)
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Data
2011-01
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Resumo
The morphofunctional aspects of oogenesis of Poecilia vivipara were studied aiming to
understand the reproductive biology and development of species with internal fertilization,
particularly those belonging to the family Poeciliidae. The stages of gonadal maturation and
follicular development were characterized using mesoscopic, histological, histochemical,
and lectin cytochemical analyses. Through mesoscopic evaluation the ovarian development
was classified in six phases of development: immature, in maturation I, in maturation II,
mature I, mature II, and post-spawn. Based on microscopic examination of the ovaries, we
identified the presence of oocytes types I and II during the previtellogenic phase and types
III, IV, and V during the vitellogenic phase. As oogenesis proceeded the oocyte cytosol
increased in volume and presented increased cytoplasmic granule accumulation,
characterizing vitellogenesis. The zona radiata (ZR) increased in thickness and complexity,
and the follicular epithelium, which was initially thin and consisting of pavimentous cells, in
type III oocytes exhibited cubic simple cells. The histochemical and cytochemical analyses
revealed alterations in the composition of the molecular structures that form the ovarian
follicle throughout the gonadal development. Our study demonstrated differences in the
female reproductive system among fish species with internal and external fertilization and
we suggest P. vivipara can be used as experimental model to test environmental toxicity.
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Ovarian development, Protein, Histology, Reproduction, Ovoviviparity, Desenvolvimento ovariano, Proteína, Histologia, Reprodução, Ovoviviparidade
Citação
ROCHA, Thiago L.; YAMADA, Áureo T.; COSTA, Renato Mazaro e; SABÓIA-MORAIS, Simone M. T. Analyses of the development and glycoproteins present in the ovarian follicles of Poecilia vivipara (Cyprinodontiformes, Poeciliidae). Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 31, n. 1, p. 87-93, Jan. 2011.