Utilização do cultivo in vitro de folículos pré-antrais suínos inclusos em tecido ovariano (in situ) como modelo de avaliação da eficiência de medicamentos homeopáticos na foliculogênese inicial

Abstract

This study investigated the effect of homeopathic medicine (Pulsatilla nigricans and homeopathic follicle stimulating hormone – FSH) and the complex (Bos Stress Fertilis) on the initial folliculogenesis, using the in vitro culture of preantral follicles as in vitro model. For this, swine ovarian fragments were cultured for one or seven days in α-MEM+ in the absence (cultured control) or presence of homeopathic FSH (6 cH), Pulsatilla (6 CH), Bos Stress Fertilis (6 CH), grain alcohol (50% v / v) or recombinant FSH (50 ng / ml) added daily. Uncultured ovarian fragments (fresh control) or cultured for one and seven days were processed for classical histology. Only the homeopathic complex Bos Stress Fertilis maintained the percentage of follicular survival after seven days of culture in relation to the uncultured control and α-MEM+. Regarding follicular growth, only the addition of homeopathic FSH increased the follicular diameter when compared to the uncultured control and a-MEM+. Thus, it can be concluded that the addition of the homeopathic remedies Bos Stress fertilis and homeopathic FSH (6 CH) improved, respectively, survival and in vitro growth of swine preantral follicles included in ovarian tissue.