Employing proteomic analysis to compare Paracoccidioides lutzii yeast and mycelium cell wall proteins
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2017
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Resumo
Paracoccidioidomycosis is an important systemic mycosis caused by thermodimorphic fungi of the
Paracoccidioides genus. During the infective process, the cell wall acts at the interface between the fungus and the
host. In this way, the cell wall has a key role in growth, environment sensing and interaction, as well as morphogenesis of the fungus. Since the cell wall is absent in mammals, it may present molecules that are described as
target sites for new antifungal drugs. Despite its importance, up to now few studies have been conducted employing proteomics in for the identification of cell wall proteins in Paracoccidioides spp. Here, a detailed proteomic approach, including cell wall-fractionation coupled to NanoUPLC-MSE
, was used to study and compare
the cell wall fractions from Paracoccidioides lutzii mycelia and yeast cells. The analyzed samples consisted of cell
wall proteins extracted by hot SDS followed by extraction by mild alkali. In summary, 512 proteins constituting
different cell wall fractions were identified, including 7 predicted GPI-dependent cell wall proteins that are
potentially involved in cell wall metabolism. Adhesins previously described in Paracoccidioides spp. such as
enolase, glyceraldehyde-3-phosphate dehydrogenase were identified. Comparing the proteins in mycelium and
yeast cells, we detected some that are common to both fungal phases, such as Ecm33, and some specific proteins,
as glucanase Crf1. All of those proteins were described in the metabolism of cell wall. Our study provides an
important elucidation of cell wall composition of fractions in Paracoccidioides, opening a way to understand the
fungus cell wall architecture
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Cell wall proteins, Paracoccidioides lutzii, Mycelia, Yeast cells, Proteomics, Paracoccidioidomycosis
Citação
ARAÚJO, Danielle Silva et al. Employing proteomic analysis to compare Paracoccidioides lutzii yeast and mycelium cell wall proteins. Biochimica et Biophysica Acta-Proteins and Proteomics, Amsterdam, v. 1865, n. 11, p. 1304-1314, 2017. Part A. DOI: 10.1016/j.bbapap.2017.08.016. Disponível em: https://www.sciencedirect.com/science/article/pii/S1570963917302029. Acesso em: 9 ago. 2024.