Resistance profile from staphylococcus aureus and pseudomonas aeruginosa obtained from tracheostomized children in the four seasons of the year
Nenhuma Miniatura disponível
Data
2022
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
Objective: The objective of this study was to microbiologically characterize the tracheal
secretion of tracheostomized children, evaluate biofilm formation and study the
phenotypic and molecular profile of the antimicrobial resistance of Sthapylococcus
aureus and Pseudomonas aeruginosa isolates. Methods: 88 samples of tracheal secretions
were collected. The material was processed to perform phenotypic tests and bacterial
identification. Tests were used to identify biofilms using Congo red agar test and
microdilution in a 96-well plate, and the qPCR method was used to verify resistance.
Results: Twelve Staphylococcus aureus samples and 30 Pseudomonas aeruginosa were
isolated of pediactric tracheostomized patients. All the S. aureus samples were positive
to biofilm formation in Congo red agar test. In the antibiogram test, S. aureus showed
resistance to seven antimicrobials. Regarding the identification of resistance genes, blaZ
was amplified in 57.1% and mecA in 28.6% of the isolated S. aureus. Pseudomonas
aeruginosa showed blaOXA with 66,7% e blaKPC with 58,3%. In plasmid DNA,
blaNDM stood out with 58,3% positive. Conclusions: The control of resistant bacteria
involved in biofilms in the stoma of tracheostomized patients is a great challenge, since the simple cannula change does not always allow the control of the microbiota, which
increases the vulnerability of patients to future respiratory complications.
Descrição
Palavras-chave
Agentes antibacterianos, Resistência, Beta-lactamases, Pediatria, Biofilme, Anti-bacterial agents, Resistance, Beta-lactamases, Pediatrics, Biofilm
Citação
ALMEIDA, Juliana Afonso de et al. Resistance profile from staphylococcus aureus and pseudomonas aeruginosa obtained from tracheostomized children in the four seasons of the year. Brazilian Journal of Development, São José dos Pinhais, v. 8, p. 81108-81133, 2022. DOI: 10.34117/bjdv8n12-285. Disponível em: https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/55835. Acesso em: 3 jun. 2025.