Resistance profile from staphylococcus aureus and pseudomonas aeruginosa obtained from tracheostomized children in the four seasons of the year

Abstract

Objective: The objective of this study was to microbiologically characterize the tracheal secretion of tracheostomized children, evaluate biofilm formation and study the phenotypic and molecular profile of the antimicrobial resistance of Sthapylococcus aureus and Pseudomonas aeruginosa isolates. Methods: 88 samples of tracheal secretions were collected. The material was processed to perform phenotypic tests and bacterial identification. Tests were used to identify biofilms using Congo red agar test and microdilution in a 96-well plate, and the qPCR method was used to verify resistance. Results: Twelve Staphylococcus aureus samples and 30 Pseudomonas aeruginosa were isolated of pediactric tracheostomized patients. All the S. aureus samples were positive to biofilm formation in Congo red agar test. In the antibiogram test, S. aureus showed resistance to seven antimicrobials. Regarding the identification of resistance genes, blaZ was amplified in 57.1% and mecA in 28.6% of the isolated S. aureus. Pseudomonas aeruginosa showed blaOXA with 66,7% e blaKPC with 58,3%. In plasmid DNA, blaNDM stood out with 58,3% positive. Conclusions: The control of resistant bacteria involved in biofilms in the stoma of tracheostomized patients is a great challenge, since the simple cannula change does not always allow the control of the microbiota, which increases the vulnerability of patients to future respiratory complications.