Novel method for the extraction of cocaine from oral fluid by means of disposable pipette modified with restricted access material

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dc.creatorTavares, Ludmyla Santos
dc.creatorBernardo, Ricardo Alves
dc.creatorOliveira, Anselmo Elcana de
dc.creatorVaz, Boniek Gontijo
dc.creatorChaves, Andréa Rodrigues
dc.date.accessioned2023-05-30T15:44:19Z
dc.date.available2023-05-30T15:44:19Z
dc.date.issued2021
dc.description.resumoThis study proposes a new, efficient, and selective method for the analysis of cocaine in oral fluid. For this purpose, an extractive phase with restricted access materials (RAM) capable of extract and pre-concentrate low molecular weight analytes and simultaneously exclude macromolecules was developed for disposable pipette extraction (DPX) followed by liquid chromatography coupled to mass spectrometry (LC-MS) analyses. The proposed method was optimized using a 24 factorial design which pointed higher extraction efficiency under the following conditions: pH 9, 10 cycles of extraction (aspirate/dispense), 3 cycles of desorption (aspirate/dispense) and acetonitrile as desorption solvent. The DPX-RAM/LC-MS method showed linearity range from 10 to 100 ng mL-1 with determination coefficient (R2) = 0.999, accuracy values ranged from -0.9 to 4.3%, precision values ranged from 1.7 to 9.1% and recovery values ranged from 97.9 to 101.1%. The benefits of this method are that it can be performed in few minutes and that the analytical performances, including the limit of detection (3.13 ng mL-1), limit of quantitation (10 ng mL-1) and linear range were found to be in agreement with other methods used for similar analysis.pt_BR
dc.identifier.citationTAVARES, Ludmyla S. et al. Novel method for the extraction of cocaine from oral fluid by means of disposable pipette modified with restricted access material. Journal of the Brazilian Chemical Society, Campinas, v. 32, n. 12, p. 2235-2244, 2021. DOI: 10.21577/0103-5053.20210115. Disponível em: https://jbcs.sbq.org.br/default.asp?ed=320. Acesso em: 17 maio 2023.pt_BR
dc.identifier.doihttps://dx.doi.org/10.21577/0103-5053.20210115
dc.identifier.issne-1678-4790
dc.identifier.issn0103-5053
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/22637
dc.language.isoengpt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentInstituto de Química - IQ (RMG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectCocainept_BR
dc.subjectRestricted access materialspt_BR
dc.subjectDisposable pipette extractionpt_BR
dc.subjectDesorptionpt_BR
dc.subjectValidationpt_BR
dc.titleNovel method for the extraction of cocaine from oral fluid by means of disposable pipette modified with restricted access materialpt_BR
dc.typeArtigopt_BR

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