Identification and characterization of Paracoccidioides lutzii proteins interacting with macrophages
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2019
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Paracoccidioidomycosis (PCM), caused by thermodimorphic fungi of the Paracoccidioides genus, is a
systemic disorder that involves the lungs and other organs. The adherence of pathogenic microorganisms
to host tissues is an essential event in the onset of colonization and spread. The hostepathogen inter action is a complex interplay between the defense mechanisms of the host and the efforts of pathogenic
microorganisms to colonize it. Therefore, the identification of fungi proteins interacting with host pro teins is an important step understanding the survival strategies of the fungus within the host. In this
paper, we used affinity chromatography based on surface proteomics (ACSP) to investigate the in teractions of pathogen proteins with host surface molecules. Paracoccidioides lutzii extracts enriched of
surface proteins were captured by chromatographic resin, which was immobilized with macrophage cell
surface proteins, and identified by mass spectrometry. A total of 215 proteins of P. lutzii were identified
interacting with macrophage proteins. In silico analysis classified those proteins according to the pres ence of sites for N- and O-glycosylation and secretion by classical and non-classical pathways. Serine
proteinase (SP) and fructose-1,6-bisphosphate aldolase (FBA) were identified in our proteomics analysis.
Immunolocalization assay and flow cytometry both showed an increase in the expression of these two
proteins during hostepathogen interaction.
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Paracoccidioides lutzii, Macrophages, Interaction, Serine proteinase, Fructose-1,6-Bisphosphate aldolase
Citação
TOMAZETT, Mariana Vieira et al. Identification and characterization of Paracoccidioides lutzii proteins interacting with macrophages. Microbes and Infection, Paris, v. 21, n. 8/9, p. 401-411, 2019. DOI: 10.1016/j.micinf.2019.03.002. Disponível em: https://www.sciencedirect.com/science/article/pii/S1286457919300462?via%3Dihub. Acesso em: 22 nov. 2024.