Protective effectiveness of an immunization protocol against the toxic effects of Loxosceles intermedia Venom in Rabbits
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Data
2022-05
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Resumo
Loxosceles spp. (brown spiders) bites are responsible for the development of a syndrome
consisting mainly of dermonecrotic lesions, and also systemic effects. Rabbits are one
of the main experimental models used for better understanding the systemic and local
effects of Loxosceles venom. The aim of this study is to evaluate the toxic and protective
effects of rabbits immunized with Loxosceles spp. venom. Male New Zealand rabbits
were allocated as a control group (CG; n = 5) that received adjuvant (Montanide)
and phosphate-buffer saline (PBS), or as venom group (VG; n = 5) that received 21
µg of Loxosceles venom using Montanide as adjuvant. After five immunization cycles,
a trial with 7 µg of Loxosceles intermedia (L. intermedia) venom was performed,
and dermonecrotic lesions were measured. The rabbits were then euthanized, and
their organs were collected for histopathology analysis. Rabbits that had undergone
Loxosceles venom immunization protocol showed minor clinical disturbances during the
experimental period. The used immunization protocol protected the rabbits against the
toxic effect of the Loxosceles venom because they showed minor clinical disturbances
during the experimental period.
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Brown spider, Dermonecrosis, Immunization, Loxoscelism, Spider bite
Citação
MIRANDA, Ana Luísa Soares de et al. Protective effectiveness of an immunization protocol against the toxic effects of loxosceles intermedia venom in rabbits. Frontiers in Veterinary Science, [s. l.], v. 9, e852917, 2022. DOI: 10.3389/fvets.2022.852917. Disponível em: https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2022.852917/full. Acesso em: 15 maio 2025.