From live cells to Caenorhabditis elegans: selective staining and quantification of lipid structures using a fluorescent hybrid benzothiadiazole derivative
| dc.creator | Mota, Alberto de Andrade Reis | |
| dc.creator | Corrêa, José Raimundo | |
| dc.creator | Andrade, Lorena Pereira de | |
| dc.creator | Assumpção, José Antonio Fagundes | |
| dc.creator | Cintra, Giovana Aparecida de Souza | |
| dc.creator | Freitas Junior, Lucio Holanda Gondim de | |
| dc.creator | Silva, Wender Alves | |
| dc.creator | Oliveira, Heibbe Cristhian Benedito de | |
| dc.creator | Silveira Neto, Brenno Amaro da | |
| dc.date.accessioned | 2024-01-26T13:14:27Z | |
| dc.date.available | 2024-01-26T13:14:27Z | |
| dc.date.issued | 2018-04-05 | |
| dc.description.abstract | The current article describes the synthesis, characterization, and application of a designed hybrid fluorescent BTD–coumarin (2,1,3-benzothiadiazole-coumarin) derivative (named BTD-Lip). The use of BTD-Lip for live-cells staining showed excellent results, and lipid droplets (LDs) could be selectively stained. When compared with the commercially available dye (BODIPY) for LD staining, it was noted that the designed hybrid fluorescence was capable of staining a considerable larger number of LDs in both live and fixed cells (ca. 40% more). The new dye was also tested on live Caenorhabditis elegans (complex model) and showed an impressive selectivity inside the worm, whereas the commercial dye showed no selectivity in the complex model. | |
| dc.identifier.citation | MOTA, Alberto A. R. et al. From live cells to Caenorhabditis elegans: selective staining and quantification of lipid structures using a fluorescent hybrid benzothiadiazole derivative. ACS Omega, Washington, v. 3, n. 4, p. 3874-3881, 2018. DOI: 10.1021/acsomega.8b00434. Disponível em: https://pubs.acs.org/doi/10.1021/acsomega.8b00434. Acesso em: 14 dez. 2023. | |
| dc.identifier.doi | 10.1021/acsomega.8b00434 | |
| dc.identifier.issn | e- 2470-1343 | |
| dc.identifier.uri | http://repositorio.bc.ufg.br//handle/ri/24203 | |
| dc.language.iso | eng | |
| dc.publisher.country | Estados unidos | |
| dc.publisher.department | Instituto de Química - IQ (RMG) | |
| dc.rights | Acesso Aberto | |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
| dc.title | From live cells to Caenorhabditis elegans: selective staining and quantification of lipid structures using a fluorescent hybrid benzothiadiazole derivative | |
| dc.type | Artigo |