Screening for glycosylphosphatidylinositol- anchored proteins in the Paracoccidioides brasiliensis transcriptome
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Data
2005
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Resumo
Open reading frames in the transcriptome of Paracoc-
cidioides brasiliensis were screened for potential glycosylphosphati-
dylinositol (GPI)-anchored proteins, which are a functionally and struc-
turally diverse family of post-translationally modified molecules found in
a variety of eukaryotic cells. Numerous studies have demonstrated that
various GPI anchor sequences can affect the localization of these pro-
teins in the plasma membrane or the cell wall. The GPI anchor core is
produced in the endoplasmic reticulum by sequential addition of monosac-
charides and phospho-ethanolamine to phosphatidylinositol. The com-
plete GPI anchor is post-translationally attached to the protein carboxyl-
terminus by GPI transamidases. Removal of this GPI lipid moiety by
phospholipases generates a soluble form of the protein. The identifica-
tion of putative GPI-attached proteins in the P. brasiliensis transcrip-
tome was based on the following criteria: the presence of an N-terminal
signal peptide for secretion and a hydrophobic region in the C-terminus
presenting the GPI-attachment site. The proteins that were identified
were in several functional categories: i) eight proteins were predicted to
be enzymes (Gel1, Gel2, Gel3, α-amylase, aspartic proteinase, Cu-Zn SOD, DFG5, PLB); ii) Ag2/PRA, ELI-Ag1 and Gel1 are probably sur-
face antigens; iii) Crh-like and the GPI-anchored cell wall protein have a
putative structural role; iv) ECM33 and Gels (1, 2 and 3) are possibly
involved in cell wall biosynthesis, and v) extracellular matrix protein is
considered to be an adhesion protein. In addition, eight deduced proteins
were predicted to localize in the plasma membrane and six in the cell
wall. We also identified proteins involved in the synthesis, attachment
and cleaving of the GPI anchor in the P. brasiliensis transcriptome.
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Palavras-chave
Paracoccidioides brasiliensis, GPI-anchored proteins, Plasma membrane, Cell wall
Citação
CASTRO, Nadya da Silva; MAIA, Zilma Alves; PEREIRA, Maristela; SOARES, Célia Maria de Almeida. Screening for glycosylphophatidylinositol (GPI)-anchored proteins in Paracoccidioides brasiliensis. Genetics and Molecular Research, Ribeirão Preto, v. 4, n. 2, p. 326-345, June 2005.