Hypomethylation of tumor suppressor genes in odontogenic myxoma
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Data
2011
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Resumo
Odontogenic myxoma (OM) is an ectomesenchymal benign odontogenic tumor characterized by spindle or stellate-shaped cells
embedded in an abundant myxoid or mucoid extracellular matrix. DNA methylation is characterized by the addition of methyl groups
in cytosines within CpG islands in the promoter gene. DNA methylation can decrease the expression of tumor suppressor genes and
contribute to the development of neoplastic lesions. The aim of study was to evaluate the methylation pattern of the tumor suppressor
genes P16 (CDKN2A), P21 (CDKN1A), P27 (CDKN1B), P53 (TP53) and RB1 in OM and dental pulp. Methylation was evaluated
using methylation-specific polymerase chain reaction (PCR). The transcription was studied in some cases by using reverse transcription
quantitative PCR. A higher frequency of unmethylated P27, P53, and RB1 samples was observed in the OM when compared with the
dental pulp. OM expressed mRNA of all the genes evaluated. Considering all the samples together, the expression of Rb was higher in
the unmethylated samples compared with the partially methylated samples. This investigation revealed hypomethylation of the genes
P27, P53, and RB1 in OM. In addition, methylation of tumor suppressor genes was found to be an usual event in normal dental pulp.
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Palavras-chave
Methylation, Dental pulp, Tumor suppressor genes, Odontogenic myxoma
Citação
MOREIRA, Paula Rocha et al. Hypomethylation of tumor suppressor genes in odontogenic myxoma. Brazilian Dental Journal, Ribeirão Preto, v. 22, n. 5, p. 422-427, 2011.