Plasma membrane rigidity effects of 4-hydroxy-2-nonenal in Leishmania, erythrocyte and macrophage

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dc.creatorAlonso, Lais
dc.creatorMenegatti, Ricardo
dc.creatorDorta, Miriam Cristina Leandro
dc.creatorAlonso, Antonio
dc.date.accessioned2023-11-24T14:45:52Z
dc.date.available2023-11-24T14:45:52Z
dc.date.issued2022
dc.description.abstract4-hydroxy-2-nonenal (HNE) is a reactive aldehyde produced by cells under conditions of oxidative stress, which has been shown to react with proteins and phosphatidylethanolamine in biological membranes. Using electron paramagnetic resonance (EPR) spectroscopy of a spin label it was demonstrated that 2 h of treatment with HNE causes membrane rigidity in promastigotes of Leishmania (L.) amazonensis, J774.A1 macrophages and erythrocytes. Remarkable fluidity-reducing effects on the parasite membrane were observed at HNE concentrations approximately 4-fold lower than in the case of erythrocyte and macrophage membranes. Autofluorescence of the parasites in PBS suspension (1 × 107 cell/mL) with excitation at 354 nm showed a linear increase of intensity in the range of 400 to 600 nm over 3 h after treatment with 30 μM HNE. Parasite ghosts prepared after this period of HNE treatment showed a high degree of membrane rigidity. Bovine serum albumin (BSA) in PBS treated with HNE for 2 h showed an increase in molecular dynamics and suffered a decrease in its ability to bind a lipid probe. In addition, the antiproliferative activity of L. amazonensis promastigotes, macrophage cytotoxicity and hemolytic potential were assessed for HNE. An IC50 of 24 μM was found, which was a concentration > 10 times lower than the cytotoxic and hemolytic concentrations of HNE. These results indicate that the action of HNE has high selectivity indices for the parasite as opposed to the macrophage and erythrocyte.
dc.identifier.citationALONSO, Lais et al. Plasma membrane rigidity effects of 4-hydroxy-2-nonenal in Leishmania, erythrocyte and macrophage. Toxicology in Vitro, Amsterdam, v. 79, e105294, 2022. DOI: 10.1016/j.tiv.2021.105294. Disponível em: https://www.sciencedirect.com/science/article/pii/S0887233321002198. Acesso em: 12 set. 2023.
dc.identifier.doi10.1016/j.tiv.2021.105294
dc.identifier.issn0887-2333
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0887233321002198
dc.language.isoeng
dc.publisher.countryHolanda
dc.publisher.departmentInstituto de Física - IF (RMG)
dc.rightsAcesso Restrito
dc.subject4-hydroxy-2-nonenalLeishmaniaMacrophagesErythrocyteMembrane fluidityelectron paramagnetic resonance
dc.subjectLeishmania
dc.subjectMacrophages
dc.subjectErythrocyte
dc.subjectMembrane fluidity
dc.subjectElectron paramagnetic resonance
dc.titlePlasma membrane rigidity effects of 4-hydroxy-2-nonenal in Leishmania, erythrocyte and macrophage
dc.typeArtigo

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