Three Prime Repair Exonuclease 1 (TREX1) expression correlates with cervical cancer cells growth in vitro and disease progression in vivo
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2019
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Alterations in specifc DNA damage repair mechanisms in the presence of human papillomavirus
(HPV) infection have been described in diferent experimental models. However, the global efect of
HPV on the expression of genes involved in these pathways has not been analyzed in detail. In the
present study, we compared the expression profle of 135 genes involved in DNA damage repair among
primary human keratinocytes (PHK), HPV-positive (SiHa and HeLa) and HPV-negative (C33A) cervical
cancer derived cell lines. We identifed 9 genes which expression pattern distinguishes HPV-positive
tumor cell lines from C33A. Moreover, we observed that Three Prime Repair Exonuclease 1 (TREX1)
expression is upregulated exclusively in HPV-transformed cell lines and PHK expressing HPV16 E6
and E7 oncogenes. We demonstrated that TREX1 silencing greatly afects tumor cells clonogenic
and anchorage independent growth potential. We showed that this efect is associated with p53
upregulation, accumulation of subG1 cells, and requires the expression of E7 from high-risk HPV
types. Finally, we observed an increase in TREX1 levels in precancerous lesions, squamous carcinomas
and adenocarcinomas clinical samples. Altogether, our results indicate that TREX1 upregulation
is important for cervical tumor cells growth and may contribute with tumor establishment and
progression.
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PRATI, Bruna et al. Three Prime Repair Exonuclease 1 (TREX1) expression correlates with cervical cancer cells growth in vitro and disease progression in vivo. Scientific Reports, London, v. 9, n. 1, e351, 2019. DOI: 10.1038/s41598-018-37064-x. Disponível em: https://www.nature.com/articles/s41598-018-37064-x. Acesso em: 14 abr. 2025.