Análises anatomopatológica e imuno-histoquímica do baço e linfonodo de cães soropositivos para leishmaniose em testes sorológicos
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Data
2021-08-25
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Universidade Federal de Goiás
Resumo
Canine leishmaniasis (CanL) is a zoonosis caused by theprotozoan of the species Leishmania infantum. The spleen and
lymph nodes undergo morphological changes during CanL. This
research aimed to perform an anatomopathological and
immunohistochemical study of these organs in dogs reactive to
leishmaniasis in the Dual-path Platform chromatographic
immunoassay (DPP®) and Enzyme Immunoabsorption Assay
(ELISA). Twenty-seven dogs were evaluated for
anatomopathological examination with 92.6% showing changes
at gross evaluation, specially splenomegaly and
lymphadenomegaly. All dogs showed changes in the spleen
unrelated to the parasitic load, with granulomatous splenitis
being the most severe change. Diffuse cortical and paracortical
hyperplasia, and hyperplasia and hypertrophy of the medullary
cords were observed in the lymph node. Amastigote forms of
Leishmania spp. were found in the spleen and lymph node at
histopathological and immunohistochemical evaluations, with
good agreement between these evaluations (k = 0.55, p =
0.00124), but no difference was observed in the parasitic
intensity of these organs at immunohistochemistry (p = 0.23). It
was concluded that spleen and lymph node from dogs reactive
to leishmaniasis on the DPP® and ELISA tests show
histomorphological changes resulting from the disease,
independent to the parasitic load, as well as these organs show
similar parasitic load at immunohistochemical test
Descrição
Palavras-chave
Amastigota, Histopatologia, Imunocoloração, Leishmania spp, Tecido linfoide, amastigote, Histopathology, Leishmania spp., Immunostaining, Lymphoid tissue
Citação
MAGALHÃES, Aline Oliveira de et al. Análises anatomopatológica e imuno-histoquímica do baço e linfonodo de cães soropositivos para leishmaniose em testes sorológicos. Ciência Animal Brasileira, Goiânia, v. 22, p. e-68909, ago. 2021. Disponível em: https://www.revistas.ufg.br/vet/article/view/6.