Efeito do laser As-Ga-AI de baixa potência sobre o músculo gastrocnêmio de rato submetido à lesão mecânica
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Data
2007-12
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Editor
Marize Campos Valadares
Resumo
The muscle is a biological tissue that possesses plasticity, and presents a high degree of remodeling
facing several stimulations. It is also frequently affected by normal-life and sports-related traumas. In this manner,
this tissue’s fast recovery, maintaining its mechanic properties is of extreme importance to the individual. Several
studies indicate the laser’s efficiency regarding the modulation of the inflammatory response in skin, tendon,
muscle and bone tissues. The objective of this work was to evaluate the effects the As-Ga-Al laser radiation has
regarding the reparation of the gastrocnemius muscle of rats after a mechanic trauma. Forty-eight rats from the
Wistar lineage were utilized, from which half were sacrificed at eight days and the remaining half at 15 days from
the beginning of the experiment. The animals were separated into the following groups, each containing eight
animals each: two control groups, two only lesioned groups and two lesioned and treated groups. The
gastrocnemius muscles of the lesioned group animals were submitted to a trauma, through an impact caused by a
mechanic trauma-inducing device. The lesioned and treated group animals received laser irradiation with 10 J/cm²
at day 1, 3, 5, and 7 following the lesioning. The lesioned group animals were not irradiated, but submitted to the
same procedures, only with the As-Ga-Al laser device turned off. At the day of the sacrifice, the animals were given
anesthetics (Rompum® and Ketalar®) using a dosage of 0,10 mL/100g of bodily mass, and were then decapitated.
The gastrocnemius muscles were retrieved, fixated in formaldehyde at 10%, processed, included in paraffin,
sectioned and colored with hematoxillin/eosin. Some of the slides were colored with picrosirius red and fast green
in order to observe the collagen. There were no alterations in the inflammatory process regarding the number of
polymorph nuclear cells, fibroblasts, macrophages and monocytes, in comparison to the control groups. The
histomorphometric assessment analysis demonstrated an improvement in the collagen tissue distribution in the
lesioned and treated groups and a decrease in collagen levels in the lesioned and treated groups that suffered
sacrifice at 15 days. Thus, laser therapy in the models utilized in this experiment may be useful in the treatment of
muscular lesions, for it promotes a decrease in collagen during the latest stage of tissue reparation, therefore
inducing a more adequate rearranging of this tissue. This therapeutic resource prevents the excess of collagen and
also muscular retractions, making way for a more adequate and precocious recovery of muscular activity.
Descrição
Palavras-chave
Sistema músculo-esquelético, Colágeno, Lasers, Musculoskeletal system, Collagen, Lasers
Citação
LUZ, Marcelo Marcos Medeiros; LUZ, Fernanda Girotto Ribeiro; SILVA, Maria de Fátima Rodrigues da; OLIVEIRA, Cláudia Santos; MATA, Fabiana Ribeiro da; MATA, João Roberto da. Efeito do laser As-Ga-AI de baixa potência sobre o músculo gastrocnêmio de rato submetido à lesão mecânica. Revista Eletrônica de Farmácia, Goiânia, v. 7, n. 2, p. 159-168, jun./dez. 2007. Disponível em: <https://revistas.ufg.br/REF/article/view/3057/3093>.