Isolation and characterization of microsatellite loci from the tick Amblyomma aureolatum (Acari: Ixodidae)
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Data
2014-11
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Fundação de Pesquisas Científicas de Ribeirão Preto
Resumo
Amblyomma aureolatum (Pallas) is the main vector of the bacterium Rickettsia rickettsii, the etiological agent of Brazilian spotted fever. This disease is the most lethal human spotted fever rickettsiosis in the world. Microsatellite loci were isolated from a dinucleotide-enriched library produced from A. aureolatum sampled in Southeastern Brazil. Eight polymorphic microsatellites were further characterized among 38 individuals sampled from São Paulo metropolitan region. The number of observed alleles ranged from 2 to 9, observed heterozygosity was 0.184-0.647, and expected heterozygosity was 0.251-0.747. Cross-species amplifications suggested that these loci will be useful for other Amblyomma species.
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Amblyomma aureolatum, Enriched library, Microsatellites, Ticks
Citação
OGRZEWALSKA, M.; BAJAY, M. M.; SCHWARCZ, K.; BAJAY, S.K.; TELLES, M. P. C.; PINHEIRO, J. B.; ZUCCHI, M.I.; PINTER, A.; LABRUNA, M. B. Isolation and characterization of microsatellite loci from the tick Amblyomma aureolatum (Acari: Ixodidae). Genetics and Molecular Research, Ribeirão Preto, v. 13, n. 4, p. 9622-9627, Nov. 2014.