Detection of SARS-CoV-2 in saliva by RT-LAMP during a screening of workers in Brazil, including pre-symptomatic carriers
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Data
2021-07-07
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The coronavirus pandemic has been causing damage to many nations, as public and private
health systems deteriorate by the increasing demand. Some infected patients have culturable
severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) even though not presenting any
symptoms, and therefore, are probably able to transmit it. Correctly diagnosing and isolating
infected patients is an important step towards preventing new infections. Current diagnostic methods
rely mainly on reverse transcription quantitative polymerase chain reaction (RT-qPCR). Methods
such as reverse transcription loop-mediated isothermal amplification (RT-LAMP) have risen as
viable alternatives, as they are cheaper and require less infrastructure, they have the potential to
be applied in low-resource scenarios and even at point-of-care. Here we report a colorimetric
RT-LAMP assay capable of detecting SARS-CoV-2 in ribonucleic acid (RNA) from saliva. In
some cases, the test was able to detect viral RNA before symptom onset and even in a self-reported
asymptomatic carrier. It had a limit of detection of 300 copies per reaction and showed a sensitivity
of 80%, a specificity of 100%, a general accuracy of 99.59%, and a Cohen’s kappa of 0.887. The
possibility of detecting positive cases even before the clinical manifestation shows great potential
and can contribute to controlling the pandemic.
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Screening, Molecular diagnosis, Isothermal amplification, COVID-19 test
Citação
SANTOS, Carlos A. dos et al. Detection of SARS-CoV-2 in saliva by RT-LAMP during a screening of workers in Brazil, including pre-symptomatic carriers. Journal of the Brazilian Chemical Society, Campinas, v. 32, n. 11, p. 2071-2077, 2021. DOI: 10.21577/0103-5053.20210098. Disponível em: https://jbcs.sbq.org.br/default.asp?ed=318. Acesso em: 13 dez. 2023.