Bioluminescence imaging in Paracoccidioides spp.: a tool to monitor the infectious processes

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dc.creatorLeite, Vanessa Rafaela Milhomem Cruz
dc.creatorTomazett, Mariana Vieira
dc.creatorCurcio, Juliana Santana de
dc.creatorOliveira, Nicolau Sbaraini
dc.creatorBailão, Alexandre Melo
dc.creatorGonçales, Relber Aguiar
dc.creatorMoraes, Dayane
dc.creatorPereira, Maristela
dc.creatorVainstein, Marilene Henning
dc.creatorSchrank, Augusto
dc.date.accessioned2024-08-16T13:48:09Z
dc.date.available2024-08-16T13:48:09Z
dc.date.issued2022
dc.description.abstractThe genus Paracoccidioides comprises the species complex causing paracoccidioidomycoses (PCM). These fungi are a serious public health problem due to the long-term drug therapy, follow-up treatment, and frequent sequelae generated by the infection, such as pulmonary fibrosis. In this sense, the objective of this work was to generate bioluminescent reporter strains of Paracoccidioides spp. harboring a thermostable, red-shifted luciferase gene under the control of different constitutive promoters. The strains were generated by the integration of a species-specific codon-optimized luciferase gene upon actin or enolase promoter's control. The insertion of the constructs in Paracoccidioides brasiliensis and Paracoccidioides lutzii yeast cells were performed through Agrobacterium tumefaciens-mediated transformation. The results demonstrated the presence of several transformants harboring the luciferase gene. These transformants were further confirmed by the expression of luciferase and by the presence of the hygromycin resistance gene. Moreover, the luciferase activity could be detected in in vitro bioluminescence assays and in vivo models of infection. In general, this work presents the methodology for the construction of bioluminescent strains of Paracoccidioides spp., highlighting potential promoters and proposing an in vivo model, in which those strains could be used for the systemic study of PCM.
dc.identifier.citationCRUZ-LEITE, Vanessa Rafaela Milhomem et al. Bioluminescence imaging in Paracoccidioides spp.: a tool to monitor the infectious processes. Microbes and Infection, Amsterdam, v. 24, n. 6/7, e104975, 2022. DOI: 10.1016/j.micinf.2022.104975. Disponível em: https://www.sciencedirect.com/science/article/pii/S1286457922000454. Acesso em: 8 ago. 2024.
dc.identifier.doi10.1016/j.micinf.2022.104975
dc.identifier.issn1286-4579
dc.identifier.urihttp://repositorio.bc.ufg.br//handle/ri/25308
dc.language.isoeng
dc.publisher.countryHolanda
dc.publisher.departmentInstituto de Ciências Biológicas - ICB (RMG)
dc.rightsAcesso Aberto
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectParacoccidioides
dc.subjectBioluminescence
dc.subjectPromoters
dc.subjectATMT
dc.subjectGalleria mellonella
dc.titleBioluminescence imaging in Paracoccidioides spp.: a tool to monitor the infectious processes
dc.typeArtigo

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