A green and lean method certified by NEMI, ESA, AGREE, GAPI and BAGI for the analysis of ivermectin in injection solution for veterinary use

Abstract

The use of antiparasitics, such as ivermectin (IVE), is extremely important to public health and the economy. Quality control and analytical development are necessary to guarantee the efficacy, safety, and quality of medicines. This work covered the development and validation of a green and lean method by UV to quantify IVE in injection solution for veterinary use. UV methodology using ethanol as a diluent, a quartz cuvette and a spectrophotometer at 245 nm were used. In order to bring objectivity in relation to the greenness of the proposed method, 5 tools were used: National Environmental Method Index (NEMI), Eco-Scale Assessment (ESA), Analytical GREEnness Metric (AGREE), Green Analytical Procedure Index (GAPI), and Blue Applicability Grade Index (BAGI). The proposed method was linear in the range of 6-16 µg mL-1, precise (RSD < 5%), selective and indicative of stability by forced degradation, exact (100.07%) and robust against small and deliberate modifications. NEMI showed the 4 green quadrants, GAPI showed predominantly green and yellow quadrants, ESA, AGREE and BAGI showed scores of 93, 0.82 and 65, respectively. The method is an excellent and lean green option for evaluating f inal IVE product. It has an environmentally friendly footprint, which can be advantageously employed by pharmaceutical chemical laboratories worldwide.