Development and cross-amplification of microsatellite markers for Micrurus surinamensis (Elapidae)
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2021
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Coral snakes have restricted and specialized diets, and their semifossorial and aquatic habits make them difficult to
observe or collect. As a consequence, there is limited information about many aspects of their biology, including genetic diversity.
Microsatellite markers have been used to study genetic variability in nonmodel species, such as snakes. The aim of this work was to
develop primers for microsatellite regions of the genome of Micrurus surinamensis, to test transferability potential to Micrurus
lemniscatus and Micrurus paraensis, and to analyze the genetic diversity of those species. DNA of one specimen of M. surinamensis was
sequenced on the MiSeq platform, and sequences were searched for microsatellite regions with 10 primers designed for regions having
tetranucleotide motifs. Micrurus surinamensis (n = 12 individuals) presented from 5 to 13 alleles and expected heterozygosity ranged from
0.741 to 0.939. Eight markers were successfully transferred to M. lemniscatus (n = 38) and five to M. paraensis (n = 13). The probability of
exclusion of paternity ranged from 0.997 to 0.999 for species, and the probability of combined identity was lower in M. surinamensis (8.41
· 10-9
). These are the first microsatellite markers to be used for studies of coral snake population genetics and conservation.
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PESSOA, Anita de Moura et al. Development and cross-amplification of microsatellite markers for Micrurus surinamensis (Elapidae). Journal of Herpetology, Washington , v. 55, n. 1, p. 33-37, 2021. DOI: 10.1670/19-081. Disponível em: https://bioone.org/journals/journal-of-herpetology/volume-55/issue-1/19-081/Development-and-Cross-Amplification-of-Microsatellite-Markers-for-Micrurus-surinamensis/10.1670/19-081.full. Acesso em: 18 jun. 2023.