Lipopolysaccharide infusion up-regulates hepcidin mRNA expression in equine liver

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dc.creatorOliveira Filho, José Paes
dc.creatorBadial, Peres Ramos
dc.creatorCunha, Paulo Henrique Jorge da
dc.creatorPeiró, Juliana Regina
dc.creatorAraújo Júnior, João Pessoa
dc.creatorDivers, Thomas J.
dc.creatorWinand, Nena J.
dc.creatorBorges, Alexandre Secorun
dc.date.accessioned2018-03-20T15:23:48Z
dc.date.available2018-03-20T15:23:48Z
dc.date.issued2011
dc.description.resumoHepcidin has been found to be the key regulator of iron metabolism that leads to hypoferremia during inflammation. Recent work has shown that equine hepcidin is predominantly expressed in the liver of horses. In this study, hepcidin gene expression was determined in the liver and bone marrow of six healthy horses after IV infusion of Escherichia coli O55:B5 LPS. The IL-6 gene expression was also determined in liver and bone marrow samples. Clinical and laboratory evaluations were measured at multiple time points between 0 and 240 h post-LPS infusion (PI). Liver and bone marrow biopsies were taken immediately before (baseline) and at 6 and 18 h PI. In response to endotoxin infusion, all horses showed characteristic clinical signs of endotoxemia. Plasma iron concentration was decreased significantly from the preinfusion level at 8 h PI. Hypoferremia peak was observed at 12 h and returned to normal levels at 30 h PI. Relative realtime RT-PCR analysis showed that liver hepcidin and IL-6 mRNA expression was up-regulated at 6 h PI. Bone marrow hepcidin relative expression was not influenced by LPS infusion. In another experiment, equine monocyte cultures were stimulated with LPS (1 mg/ml). Monocyte hepcidin and IL-6 gene expression was significantly induced after 2 h of LPS stimulus and returned to baseline levels thereafter. The present study describes that, in horses, LPS infusion up-regulates hepatic hepcidin mRNA expression resulting in early observed hypoferremia and suggests that hepcidin may act as an acute-phase protein in horses.pt_BR
dc.identifier.citationOLIVEIRA FILHO, José P.; BADIAL, Peres R.; CUNHA, Paulo H. J.; PEIRÓ, Juliana R.; ARAÚJO JÚNIOR, João P.; DIVERS, Thomas J.; WINAND, Nena J.; BORGES, Alexandre S. Lipopolysaccharide infusion up-regulates hepcidin mRNA expression in equine liver. Innate Immunity, London, v. 18, n. 3, p. 438-446, 2011.pt_BR
dc.identifier.doi10.1177/1753425911420181
dc.identifier.issne- 17534267
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/14076
dc.language.isoengpt_BR
dc.publisher.countryGra-bretanhapt_BR
dc.publisher.departmentEscola de Veterinária e Zootecnia - EVZ (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectAcute-phase reactantspt_BR
dc.subjectEndotoxinspt_BR
dc.subjectHepcidinpt_BR
dc.subjectInflammationpt_BR
dc.subjectIron metabolismpt_BR
dc.titleLipopolysaccharide infusion up-regulates hepcidin mRNA expression in equine liverpt_BR
dc.typeArtigopt_BR

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