4-Fluorobenzaldehyde limonene-based thiosemicarbazone induces apoptosis in PC-3 human prostate cancer cells

Página do item simplificado
dc.creatorRodrigues, Bruna dos Santos
dc.creatorMarcelino, Renato Ivan de Ávila
dc.creatorMiranda, Polyana Lopes Benfica
dc.creatorPires, Ludmila Bringel
dc.creatorOliveira, Cecilia Maria Alves de
dc.creatorVandresen, Fábio
dc.creatorSilva, Cleuza Conceição da
dc.creatorValadares, Marize Campos
dc.date.accessioned2023-09-05T10:31:18Z
dc.date.available2023-09-05T10:31:18Z
dc.date.issued2018
dc.description.abstractAims: This study evaluated parameters of toxicity and antiproliferative effects of (+)-N(1)-4-fluorobenzaldehyde-N(4)-{1-methyl-1-[(1R)-4-methylcyclohexene-3-il]-ethyl}-thiossemicarbazone (4-FTSC) in PC-3 adenocarcinoma prostate cells. Main methods: Cytotoxicity of 4-FTSC in PC-3 cells was evaluated using MTT assay. Morphology examination of PC-3 cells treated with 4-FTSC was also performed as well as the cell death mechanisms induced were investigated using flow cytometry. Parameters of toxicity of 4-FTSC was conducted by the investigation of its potential myelotoxicity and lymphotoxicity, hemolytic activity and acute oral toxicity profile. Key findings: 4-FTSC showed promising cytotoxic effects against PC-3 cells (IC50 = 18.46 μM). It also triggered apoptotic morphological changes, phosphatidylserine externalization and a significant increase of DNA fragmentation in PC-3 cells. Moreover, 4-FTSC did not show changes in the PC-3 cell cycle with levels of p21, p27, NFĸB and cyclin D1 similar to those found in both control and treated cells. 4-FTSC also promoted an increase of p53 levels associated with mitochondrial impairment through loss of ∆Ψm and ROS overproduction. 4-FTSC-induced cell death mechanism in PC-3 cells involved activation of caspase-3/-7 through apoptosis intrinsic pathway via caspase-9. Regarding toxicological profile, 4-FTSC showed in vitro lymphotoxicity, although with low cytotoxicity for bone marrow progenitors and no hemolytic potential. Moreover, it was classified as GHS category 5 (LD50 > 2000–5000 mg/Kg), suggesting it has low acute oral systemic toxicity. Significance: 4-FTSC seems to be a promising candidate to be used as a clinical tool in prostate cancer treatment. Further studies are required to better clarify its toxicopharmacological effects found in this compound.pt_BR
dc.identifier.citationRODRIGUES, Bruna dos Santos et al. 4-Fluorobenzaldehyde limonene-based thiosemicarbazone induces apoptosis in PC-3 human prostate cancer cells. Life Sciences, Amsterdam, v. 203, p. 141-149, 2018. DOI: 10.1016/j.lfs.2018.04.024. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S0024320518302054?via%3Dihub. Acesso em: 28 ago. 2023.pt_BR
dc.identifier.doi10.1016/j.lfs.2018.04.024
dc.identifier.issne- 1879-0631
dc.identifier.issn0024-3205
dc.identifier.urihttps://www.sciencedirect.com/science/article/abs/pii/S0024320518302054?via%3Dihub
dc.language.isoengpt_BR
dc.publisher.countryHolandapt_BR
dc.publisher.departmentInstituto de Química - IQ (RMG)pt_BR
dc.rightsAcesso Restritopt_BR
dc.subjectThiosemicarbazonept_BR
dc.subjectLimonenept_BR
dc.subjectApoptosispt_BR
dc.subjectProstate cancerpt_BR
dc.title4-Fluorobenzaldehyde limonene-based thiosemicarbazone induces apoptosis in PC-3 human prostate cancer cellspt_BR
dc.typeArtigopt_BR

Arquivos

Licença do Pacote
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Baixar

Coleções

IQ - Artigos publicados em periódicos