Steroidal saponin concentrations in Brachiaria decumbens and B. brizantha at different developmental stages
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Data
2009-02
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Resumo
Brachiaria species contain steroidal saponins and
are involved in outbreaks of hepatogenous photosensitization.
This research presents the levels of a steroidal saponin,
protodioscin, in the seeds and aerial parts of B. brizantha and
B. decumbens during different developmental stages (growth,
bloom, fructification and seed fall). The butanolic fraction of
the ethanolic extract of each stage was submitted to thin layer
chromatography (TLC) and spectrophotometric analysis
through the Ehrlich reagent in 515nm. The chromatograms in
TLC of the butanolic fraction of B. brizantha and B. decumbens
showed similar spots as the protodioscin standard. The estimated
level of protodioscin isomers in B. brizantha and B. decumbens
ranged from 0.5% to 2.1%, having the highest level at the end
of their developmental stages during seed falling comparison
with the previous one. Protodioscin was not detected in the
seeds. Outbreaks of Brachiaria spp. poisoning in central Western
Brazil are frequently observed in pastures that had been more
than 30 days without animals grazing, and also during the
growing or blooming stage of the pastures. Other saponin
determinations in toxic and non toxic pastures are necessary to
determine the saponin concentrations that cause intoxication.
Descrição
Palavras-chave
Brachiaria spp., Photosensitization, Protodioscin, Spectrophotometry, Steroidal saponins, Fotossensibilização, Protodioscina, Espectrofotometria, Saponina esteroidal
Citação
BRUM, Karine Bonucielli; HARAGUCHI, Mitsue; GARUTTI, Mirella Biasoli; NÓBREGA, Fernanda Nogarol; ROSA, Beneval; FIORAVANTI, Maria Clorinda Soares. Steroidal saponin concentrations in Brachiaria decumbens and B. brizantha at different developmental stages. Ciência Rural, Santa Maria, v. 39, n. 1, p. 279-281, Jan./Feb. 2009.