Estudo da microbiota ocular como monitor do 2-1 fosfato dissódico de betametasona e do fosfato dissódico/acetato de dexametasona, em cães que receberam transplantes com córneas de suínos

Abstract

Ten mixed breed dogs, with a 10 kg average weight, were sellected Centro de Zoonoses de Goiânia. After being exanimed and considered healthy, the dogs were ennmerated from 11 to 20 on their collars. Each animal had swine cornea implanted into the left eye. The swine cornea was obtained at Casa de Banha Caçula, a slaughterhouse, and conserved in humid chamber during a maximnm 6 honr period. Before surgery, each animal received 4 mg dissodic fosphate/acetate of dexamethasone IM and one drop of betamethasone dissodic fosphate and gentamycin sulphate. This medication was kept until euthanasia. Each dog had a snperior fornix sample collected, the sample was immersed in 0.1 % peptonized water, so that tests for aerobic bacteria and fungns could be realized. Each test was repeated with samples collected on days 7,14,21 and 28, after graft, and compared with those from day O. The 12,8% Staphylococcus epidermidis on initial samples was caused by contamination during collection because it wan't found on the 28"' day samples. This fact snggests that, although corticosteroids had caused systemic imunocellular snpression, they didn 't make easier the bacterial development into the grafted eye.