Can a field molecular diagnosis be accurate? A performance evaluation of colorimetric RT-LAMP for the detection of SARS-CoV-2 in a hospital setting

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dc.creatorSilva, Lívia do Carmo
dc.creatorSantos, Carlos Abelardo dos
dc.creatorMendes, Geovana de Melo
dc.creatorOliveira, Kézia Gomes de
dc.creatorSouza Junior, Marcio Neres de
dc.creatorEstrela, Paulo Felipe Neves
dc.creatorCosta, Sérgio Henrique Nascente
dc.creatorLacerda, Elisângela de Paula Silveira
dc.creatorDuarte, Gabriela Rodrigues Mendes
dc.date.accessioned2024-01-04T14:07:01Z
dc.date.available2024-01-04T14:07:01Z
dc.date.issued2021
dc.description.abstractSARS-CoV-2 currently represents a serious global public health problem. Non-pharmaceutical intervention measures (NPIs) have been widely adopted, and the testing strategy since the beginning of the infection is the most effective tool for tracking, isolating, and minimizing transmission. The high operating costs and the need for sophisticated instrumentation related to gold standard diagnostic for COVID-19, Reverse Transcription quantitative Polymerase Chain Reaction (RT-qPCR), have highlighted the urgency and importance of developing and applying new diagnostic techniques, especially in places with scarce resources. Thus, alternative molecular tests, such as Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP), based on isothermal amplification have been used to detect SARS-CoV-2 using different protocols. The potential for field application of RT-LAMP is due to the lower cost and time and not requiring high-cost instrumentation. Here, we evaluate the colorimetric RT-LAMP to detect SARS-CoV-2 in a hospital environment and correlate its performance with tests performed in a reference laboratory. The analysis performed at the hospital showed high sensitivity (88.89%), specificity (98.55%), accuracy (95.83%), and a Cohen's kappa of 0.895. However, we achieved 100% of agreement when comparing the RT-LAMP results with the gold standard (qRT-PCR) results for samples with Ct < 30 in the hospital-based test. In addition, a similar performance was found in the field compared to the reference laboratory, corroborating the proposal to apply the test directly at point-of-care.
dc.identifier.citationSILVA, Lívia do Carmo et al. Can a field molecular diagnosis be accurate? A performance evaluation of colorimetric RT-LAMP for the detection of SARS-CoV-2 in a hospital setting. Analytical Methods, [s. l.], v. 13, n. 26, p. 2898-2907, 2021. DOI: 10.1039/D1AY00481F. Disponível em: https://pubs.rsc.org/en/content/articlelanding/2021/AY/D1AY00481F. Acesso em: 13 dez. 2023.
dc.identifier.doi10.1039/D1AY00481F
dc.identifier.issne- 1759-9679
dc.identifier.urihttps://pubs.rsc.org/en/content/articlelanding/2021/AY/D1AY00481F
dc.language.isoeng
dc.publisher.countryGra-bretanha
dc.publisher.departmentInstituto de Química - IQ (RMG)
dc.rightsAcesso Restrito
dc.titleCan a field molecular diagnosis be accurate? A performance evaluation of colorimetric RT-LAMP for the detection of SARS-CoV-2 in a hospital setting
dc.typeArtigo

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