Anti-CEA loaded maghemite nanoparticles as a theragnostic device for colorectal cancer

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dc.creatorPaz, Mariana Campos da
dc.creatorSantos, Maria de Fátima M. Almeida
dc.creatorSantos, Camila M.B.
dc.creatorSilva, Sebastiao William da
dc.creatorSouza, Lincoln Bernardo de
dc.creatorLima, Emilia Celma de Oliveira
dc.creatorSilva, Renata C.
dc.creatorLucci, Carolina Madeira
dc.creatorMorais, Paulo César
dc.creatorAzevedo, Ricardo B.
dc.creatorLacava, Zulmira Guerrero Marques
dc.date.accessioned2018-07-16T12:09:35Z
dc.date.available2018-07-16T12:09:35Z
dc.date.issued2012-10
dc.description.abstractNanosized maghemite particles were synthesized, precoated (with dimercaptosuccinic acid) and surface-functionalized with anticarcinoembryonic antigen (anti-CEA) and successfully used to target cell lines expressing the CEA, characteristic of colorectal cancer (CRC) cells. The as-developed nanosized material device, consisting of surface decorated maghemite nanoparticles suspended as a biocompatible magnetic fluid (MF) sample, labeled MF-anti-CEA, was characterized and tested against two cell lines: a high-CEA expressing cell line (LS174T) and a low-CEA expressing cell line (HCT116). Whereas X-ray diffraction was used to assess the average core size of the as-synthesized maghemite particles (average 8.3 nm in diameter), dynamic light scattering and electrophoretic mobility measurements were used to obtain the average hydrodynamic diameter (550 nm) and the zeta-potential (‑38 mV) of the as-prepared and maghemite-based nanosized device, respectively. Additionally, surface-enhanced Raman spectroscopy (SERS) was used to track the surface decoration of the nanosized maghemite particles from the very first precoating up to the attachment of the anti-CEA moiety. The Raman peak at 1655 cm‑1, absent in the free anti-CEA spectrum, is the signature of the anti-CEA binding onto the precoated magnetic nanoparticles. Whereas MTT assay was used to confirm the low cell toxicity of the MF-anti-CEA device, ELISA and Prussian blue iron staining tests performed with both cell lines (LS174T and HCT116) confirm that the as-prepared MF-anti-CEA is highly specific for CEA-expressing cells. Finally, transmission electron microscopy analyses show that the association with anti-CEA seems to increase the number of LS174T cells with internalized maghemite nanoparticles, whereas no such increase seems to occur in the HCT116 cell line. In conclusion, the MF-anti-CEA sample is a biocompatible device that can specifically target CEA, suggesting its potential use as a theragnostic tool for CEA-expressing tumors, micrometastasis, and cancer-circulating cells.pt_BR
dc.identifier.citationPAZ, Mariana Campos da et al. Anti-CEA loaded maghemite nanoparticles as a theragnostic device for colorectal cancer. International Journal of Nanomedicine, Macclesfield, v. 7, p. 5271-5282, Oct. 2012.pt_BR
dc.identifier.doi10.2147/IJN.S32139
dc.identifier.issn1176-9114
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/15407
dc.language.isoengpt_BR
dc.publisher.countryGra-bretanhapt_BR
dc.publisher.departmentInstituto de Química - IQ (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectMagnetic nanoparticlespt_BR
dc.subjectAnti-CEA antibodypt_BR
dc.subjectTargeted deliverypt_BR
dc.subjectDiagnosticpt_BR
dc.subjectRamanpt_BR
dc.subjectBiocompatible devicept_BR
dc.titleAnti-CEA loaded maghemite nanoparticles as a theragnostic device for colorectal cancerpt_BR
dc.typeArtigopt_BR

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