Dysbiosis and predicted function of dental and ruminal microbiome associated with bovine periodontitis
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2022-08
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Extensive cattle livestock is advancing in Amazonia and its low productivity,
with consequent pressure to open new areas, is partly due to sanitary
problems and, among them, the periodontal diseases, whose environmental
triggers or modifying factors are unknown. In this study, we used highthroughput sequencing, network analysis and predicted functions to
investigate the dental and ruminal microbiota of cattle raised in new livestock
areas in the Amazon and identify possible keystone pathogens and proteins
associated with the disease. Ninety-three genera were common in dental
and ruminal fluid microbiomes and among them periodontal pathogens
such as Fusobacterium, Prevotella, Porphyromonas and Actinomyces were
recognized. Network analysis showed that dental microbiomes of clinically
healthy animals tend to comprise a group of OTUs in homeostasis and when
analyzed together, dental and ruminal fluid microbiomes of animals with
periodontitis had almost twice the number of negative edges, indicating
possible competition between bacteria and dysbiosis. The incisor dental and
ruminal fluid microbiomes were dominated by a core community composed
of members of the phyla Firmicutes and Bacteroidetes. Network results
showed that members of the Prevotella genus stood out among the top five
OTUs, with the largest number of hubs in the dental and ruminal microbiota
of animals with periodontitis. Protein families linked to an inflammatory
environment were predicted in the dental and ruminal microbiota of cattle with
periodontitis. The dissimilarity between dental microbiomes, discriminating
between healthy cattle and those with periodontitis and the identification
of possible key pathogens, represent an important reference to elucidate
the triggers involved in the etiopathogenesis of bovine periodontitis, and possibly in the development of measures to control the disease and reduce
the pressures for deforestation.
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Dental microbiome, Ruminal fluid microbiome, Periodontitis, Prevotella, Bovine, Biodiversity, Amazon
Citação
BORSANELLI, Ana C. et al. Dysbiosis and predicted function of dental and ruminal microbiome associated with bovine periodontitis. Frontiers in Microbiology, [s. l.], v. 13, e936021, 2022. DOI: 10.3389/fmicb.2022.936021. Disponível em: https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.936021/full. Acesso em: 05 maio 2025.