Genetic structure and chemical diversity in natural populations of Uncaria guianensis (Aubl.) J.F.Gmel. (Rubiaceae)
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2018-10
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Uncaria guianensis is native to the Amazon and is used traditionally as an anti-inflammatory.
Natural populations of the species have declined markedly in recent times because of strong
anthropic pressure brought about by deforestation and indiscriminate collection. The aim of
the present study was to assess the genetic and chemical diversity among eight natural populations
of U. guianensis located in the Brazilian states of Acre, Amapa´ and Amazonas. A
set of four primer combinations was employed in sequence-related amplified polymorphism
(SRAP) amplifications of leaf DNA, and the fragments were analyzed in an LI-COR model
4300 DNA Analyzer. Genetic variability within the populations (81%) was substantially
greater than that detected between them (19%). The highest percentage of polymorphic loci
(90.21%) and the largest genetic variability were observed in the population located in
Mazagão, Amapa´ . Genetic differentiation between populations was high (Fst = 0.188) and
the studied populations formed three distinct genetic groups (K = 3). The population located
in Assis Brasil, Acre, presented the highest average content of the mitraphylline (0.60 mg/g
dry weight,). However, mitraphylline and isomitraphylline not detected in most individuals in
the studied populations, and it is questionable whether they should be considered as chemical
markers of the species. The genetic data confirm the urgent need for conservation programs
for U. guianensis, and for further studies aimed at ascertaining the genetic basis and
heritability of alkaloid accumulation.
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HONÓRIO, Isabela Cristina G. et al. Genetic structure and chemical diversity in natural populations of Uncaria guianensis (Aubl.) J.F.Gmel. (Rubiaceae). Plos One, San Francisco, v. 13, n. 10, e0205667, Oct. 2018. DOI: 10.1371/journal.pone.0205667. Disponível em: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0205667. Acesso em: 26 jan. 2023.