Antifungal susceptibilities of clinical and environmental isolates of Cryptococcus neoformans in Goiânia city, Goias, Brazil

Resumo

We evaluated the antifungal activities of amphotericin B, fluconazole, itraconazole and voriconazole in 70 Cryptococcus neoformans strains obtained from cerebrospinal fluid from AIDS patients and 40 C. neoformans strains isolated from the environment. Four clinical isolates were identified as C. neoformans var. gattii. The susceptibility test was done using a broth microdilution method according to NCCLS M27-A2. Range minimal inhibitory concentrations (MICs) for C. neoformans clinical isolates were 0.06-1.0 μg/mL for amphotericin B, 0.125-8 μg/mL for fluconazole, 0.03-0.5 μg/mL for itraconazole and 0.03-0.25 μg/mL for voriconazole. C. neoformans environmental isolates showed range MICs 0.015-0.125 μg/mL, 0.25-2.0 μg/mL, 0.007-0.125 μg/mL and 0.03-0.25 μg/mL for amphotericin B, fluconazole, itraconazole and voriconazole respectively. The MICs results obtained from clinical and environmental isolates showed similar pattern of susceptibility and no resistance has been found in our isolates.

Descrição

Palavras-chave

Cryptococcus neoformans, Environment, Antifungal agents, Cerebrospinal fluid

Citação

SOUZA, Lúcia Kioko Hasimoto; FERNANDES, Orionalda de Fátima Lisboa; KOBAYASHI, Claudia Castelo Branco Artiaga; PASSOS, Xisto Sena; COSTA, Carolina Rodrigues; LEMOS, Janine de Aquino; SOUZA-JUNIOR, Ary Henrique de; SILVA, Maria do Rosário Rodrigues. Antifungal susceptibilities of clinical and environmental isolates of Cryptococcus neoformans in Goiânia city, Goias, Brazil. Revista do Instituto de Medicina Tropical de São Paulo, São Paulo, v. 47, n. 5, p. 253-256, Sept./Oct. 2005.