Development and analytical validation of the methodology for vitamins in tablets by ultra-performance liquid chromatography
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Data
2019-10-14
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Resumo
In the present study, we developed a reliable and robust chromatographic method for the
quantification of multivitamins in tablet samples by ultra-performance liquid chromatography
(UPLC) with photodiode array detection. The vitamins nicotinamide, pyridoxine, riboflavin, and
thiamin were analyzed and quantified in a total analysis time of 2.5 minutes, using hydrophilic
interaction liquid chromatography stationary phase. Tocopherol acetate and cyanocobalamin were
analyzed and quantified in a total analysis time of 2.5 minutes, using reversed-phase (RP)-UPLC.
The analysis time reported here is lower than that of similar methods reported in the literature
for single vitamin determination. The method linearity exhibits a good correlation coefficient
(R2 = 0.998) with the relative residual standard deviation in the acceptable limit of 2.0%. The
developed methods were validated, and the results demonstrated that the proposed analytical
method showed to be selective, sensitive, accurate, and robust for the quantification of evaluated
vitamins in multivitamin tablets. The work was fully developed in the quality control laboratory of
a pharmaceutical industry in the Agroindustrial District of Anápolis (DAIA, Goiás, Brazil), where the
product is manufactured.
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UPLC, Multivitamin tablets, Hydrophilic interaction liquid chromatography, Analytical validation
Citação
D’OLIVEIRA, Giulio D. C.; CHAVES, Andréa R.; PÉREZ, Caridad N. Development and analytical validation of the methodology for vitamins in tablets by ultra-performance liquid chromatography. Journal of Chromatographic Science, Oxford, v. 57, n. 10, p. 881-891, Oct. 2019. DOI: 10.1093/chromsci/bmz070. Disponível em: https://academic.oup.com/chromsci/article/57/10/881/5586917?login=false. Acesso em: 17 maio 2023.