DNA methylation alterations induced by transient exposure of MCF-7 cells to maghemite nanoparticles
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2017
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Aim: To evaluate the DNA methylation profile of MCF-7 cells during and after the treatment with maghemite nanoparticles (MNP-CIT). Materials & methods: Noncytotoxic MNP-CIT concentrations and cell morphology were evaluated by standard methods. DNA methylation was assessed by whole genome bisulfite sequencing. DNA methyltransferase (DNMT) genes expression was analyzed by qRT-PCR. Results: A total of 30 and 60 µgFeml-1 MNP-CIT accumulated in cytoplasm but did not present cytotoxic effects. The overall percentage of DNA methylation was not affected, but 58 gene-associated regions underwent DNA methylation reprogramming, including genes related to cancer onset. DNMT transcript levels were also modulated. Conclusion: Transient exposure to MNP-CIT promoted epigenomic changes and altered the DNMT genes regulation in MCF-7 cells. These events should be considered for biomedical applications.
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DNA methylation, Epigenome reprogramming, Magnetic nanoparticles, MCF-7 cells, Mransient exposure
Citação
BONADIO, Raphael S. et al. DNA methylation alterations induced by transient exposure of MCF-7 cells to maghemite nanoparticles. Nanomedicine, London, v. 12, n. 23, p. 2637-2649, 2017. DOI: 10.2217/nnm-2017-0241. Disponível em: https://www.futuremedicine.com/doi/10.2217/nnm-2017-0241. Acesso em: 30 out. 2023.