Towards a versatile and economic Chagas Disease point-of-care testing system, by integrating loop-mediated isothermal amplification and contactless/label-free conductivity detection

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dc.creatorFigueredo, Federico
dc.creatorStolowicz, Fabiana Gisela
dc.creatorVojnov, Adrián
dc.creatorColtro, Wendell Karlos Tomazelli
dc.creatorLarocca, Luciana
dc.creatorCarrillo, Carolina
dc.creatorCortón, Eduardo
dc.date.accessioned2024-11-04T17:02:11Z
dc.date.available2024-11-04T17:02:11Z
dc.date.issued2021-05-14
dc.description.abstractRapid diagnosis by using small, simple, and portable devices could represent one of the best strategies to limit the damage and contain the spread of viral, bacterial or protozoa diseases, principally when they can be transmitted by air and are highly contagious, as some respiratory viruses are. The presence of antibodies in blood or serum samples is not the best option for deciding when a person must be quarantined to stop transmission of disease, given that cured patients have antibodies, so the best diagnosis methods rely on the use of nucleic acid amplification procedures. Here we present a very simple device and detection principle, based on paper discs coupled to contactless conductivity (C4D) sensors, can provide fast and easy diagnostics that are needed when an epidemic outbreak develops. The paper device presented here solves one of the main drawbacks that nucleic acid amplification tests have when they are performed outside of central laboratories. As the device is sealed before amplification and integrally disposed in this way, amplimers release cannot occur, allowing repetitive testing in the physician’s practice, ambulances, or other places that are not prepared to avoid cross-contamination of new samples. The use of very low volume samples allows efficient reagent use and the development of low cost, simple, and disposable point-of-care diagnostic systems.
dc.identifier.citationFIGUEREDO, Federico et al. Towards a versatile and economic Chagas Disease point-of-care testing system, by integrating loop-mediated isothermal amplification and contactless/label-free conductivity detection. PLoS Neglected Tropical Diseases, San Francisco, v. 15, n. 5, e0009406, 2021. DOI: 10.1371/journal.pntd.0009406. Disponível em: https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0009406. Acesso em: 8 ago. 2024.
dc.identifier.doi10.1371/journal.pntd.0009406
dc.identifier.issne- 1935-2735
dc.identifier.urihttp://repositorio.bc.ufg.br//handle/ri/25753
dc.language.isoeng
dc.publisher.countryEstados unidos
dc.publisher.departmentInstituto de Química - IQ (RMG)
dc.rightsAcesso Aberto
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.titleTowards a versatile and economic Chagas Disease point-of-care testing system, by integrating loop-mediated isothermal amplification and contactless/label-free conductivity detection
dc.typeArtigo

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