Assessing genotoxicity and mutagenicity of three common amphibian species inhabiting agroecosystem environment
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2019
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Amphibians are constantly exposed to pollutants and the stress of agricultural activities. We selected three anuran amphibian species Dendropsophus minutus, Boana albopunctata, and Physalaemus cuvieri, totaling 309 individuals. We collected
tadpoles in 15 permanent ponds: 5 soybean crops, 3 corn crops, and 7 nonagricultural lands. Our study provides the frst
comparative data on the genotoxicity and mutagenicity of three common amphibian anurans. Dendropsophus minutus was
the most vulnerable species compared with B. albopunctata and P. cuvieri for comet assay and micronuclei test. However,
the more signifcant amount of DNA damage seen in D. minutus does not mean that their populations are threatened once
such species adapt well to anthropogenic disturbances. Despite, P. cuvieri was less sensitive than the other two species;
the DNA damage was signifcantly higher in soybean crops. Physalaemus cuvieri is a leptodactylidae species that deposit
their eggs in foam nests, which are essential to protect eggs from dehydration. Moreover, the foam reduces the contact of
eggs with water; thus, P. cuvieri eggs could be less exposed to contaminants present in pounds, compared with D. minutus
and B. albopunctata, which deposit their eggs directly in the water. Therefore, this study was sufciently sensitive to detect
genotoxic and mutagenic efects in tadpoles exposed to agroecosystems. We strongly suggest D. minutus in future biomonitoring studies that involve the comparison of anthropized versus not anthropized environments. Overall, we recommend the
comet assay and micronucleus test as efective methods for the detection of genotoxic damage in amphibian anurans to the
environmental disturbance, especially in agricultural sites.
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GONÇALVES, M. W. et al. Assessing genotoxicity and mutagenicity of three common amphibian species inhabiting agroecosystem environment. Archives of Environmental Contamination and Toxicology, Berlim, v. 77, p. 409-420, 2019. DOI: 10.1007/s00244-019-00647-4. Disponível em: https://link.springer.com/article/10.1007/s00244-019-00647-4. Acesso em: 7 jun. 2023.