Estabelecimento in vitro de duas espécies de bambu: Dendrocalamus asper (Schultes f.) Backer ex Heyne e Bambusa oldhamii Munro

Abstract

Bamboo has economic importance in many areas such as construction, manufacturing paper, energy supply, food industry, furniture, etc. Getting seedling is the main difficulty for the large-scale cultivation, in vitro culture becomes the most appropriate tool to resolve the issue. Several protocols have been established for many species of bamboo. However, the known protocols are not reproducible for different species. The aim was development of protocol for in vitro establishment and multiplication of two species with economic importance, Dendrocalamus asper (Schultes f.) Backer ex Heyne e Bambusa oldhamii Munro, to eliminate or reduce the microorganism that affect cultivation and in vitro multiplication. Decontamination methods were tested with or without antibiotic and the cytokinins effect on explant shoots from nodal segments on MS medium (MURASHIGE e SKOOG, 1962). The percentage of decontamination of explants subjected to aseptic process varied between the two species analyzed. Explants of D. asper had higher percentage of contamination. By comparing the percentage of contamination between the two treatments B. oldhamii, with or without antibiotic, was finding that use of streptomycin was effective in reducing 38.05% of bacterial contamination. Although the use of streptomycin does not interfere in the number of issued shoots, new experiments should be done to check the increase in the fungal contamination found during the experiments.