Protective effects of steroidal alkaloids isolated from Solanum paniculatum L. against mitomycin cytotoxic and genotoxic actions
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Data
2013-06
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Resumo
Solanum paniculatum L. is a plant species widespread throughout tropical America, especially in the
Brazilian Cerrado region. It is used in Brazil for culinary purposes and in folk medicine to treat liver and
gastric dysfunctions, as well as hangovers. Previous studies with S. paniculatum ethanolic leaf extract or
ethanolic fruit extract demonstrated that they have no genotoxic activity neither in mice nor in bacterial
strains, although their cytotoxicity and antigenotoxicity were demonstrated in higher doses. In order
to assess the possible compounds responsible for the activities observed, we fractionated the ethanolic
fruit extract of S. paniculatum, characterized by 1 H and 13 C NMR spectra, and evaluated two fractions
containing steroidal alkaloids against mitomycin C (MMC) using the mouse bone marrow micronucleus
test. Swiss mice were orally treated with different concentrations (25, 50, or 100 mg.kg -1 ) of each fraction
simultaneously with a single intraperitonial dose of MMC (4 mg.kg -1 ). Antigenotoxicity was evaluated by
using the frequency of micronucleated polychromatic erythrocytes (MNPCE), whereas anticytotoxicity
was assessed by the polychromatic and normochromatic erythrocytes ratio (PCE/NCE). Our results
demonstrated that steroidal alkaloids isolated from S. paniculatum strongly protected cells against MMC
aneugenic and/or clastogenic activities as well as modulated MMC cytotoxic action.
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Anticytotoxicity, Solanaceae, Antigenotoxicity, Jurubeba, Mmicronuclei, Anticitotoxicidade, Anticitotoxicidade, Jurubeba, MicronĂșcleo, Solanaceae
Citação
VIEIRA, Pabline M.; MARINHO, Lorena P. M.; FERRI, Suzana C. S.; LEE, Chen-Chen. Protective effects of steroidal alkaloids isolated from Solanum paniculatum L. against mitomycin cytotoxic and genotoxic actions. Anais da Academia Brasileira de CiĂȘncias, Rio de Janeiro, v. 85, n. 2, p. 553-560, Apr./June 2013.