Platelet-activating factor increases reactive oxygen species-mediated microbicidal activity of human macrophages infected with Leishmania (Viannia) braziliensis
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2017
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Resumo
Platelet-activating factor (PAF) is produced by macrophages during inflammation and infections. We evaluated whether PAF
is able to modulate the infection of human macrophages by Leishmania braziliensis, the main Leishmania sp. in Brazil.
Monocyte-derived macrophages were incubated with promastigote forms in absence or presence of exogenous PAF. We
observed that the treatment of macrophages with low concentrations of PAF prior to infection increased the phagocytosis
of L. braziliensis. More importantly, exogenous PAF reduced the parasitism when it was added before, during or after
infection. In addition, treatment with a PAF antagonist (PCA 4248) resulted in a significant increase of macrophage infection
in a concentration-dependent manner, suggesting that endogenous PAF is important to control L. braziliensis infection.
Mechanistically, while exogenous PAF increased production of reactive oxygen species (ROS) treatment with PCA 4248
reduced oxidative burst during L. braziliensis infection. The microbicidal effects of exogenous PAF were abolished when
macrophages were treated with apocynin, an NADPH oxidase inhibitor. The data show that PAF promotes the production of
ROS induced by L. braziliensis, suggesting that this lipid mediator may be relevant to control L. braziliensis infection in
human macrophages.
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Leishmania (Viannia) braziliensis, Human macrophage, Platelet-activating factor, Reactive oxygen species
Citação
BORGES, Arissa Felipe et al. Platelet-activating factor increases reactive oxygen species-mediated microbicidal activity of human macrophages infected with Leishmania (Viannia) braziliensis. Pathogens and Disease, Oxford, v. 75, n. 7, eftx082, 2017. DOI: 10.1093/femspd/ftx082. Disponível em: https://academic.oup.com/femspd/article/75/7/ftx082/3983173?login=true. Acesso em: 20 mar. 2025.