Platelet-activating factor increases reactive oxygen species-mediated microbicidal activity of human macrophages infected with Leishmania (Viannia) braziliensis

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dc.creatorBorges, Arissa Felipe
dc.creatorMorato, Camila Imai
dc.creatorGomes, Rodrigo Saar
dc.creatorDorta, Miriam Cristina Leandro
dc.creatorOliveira, Milton Adriano Pelli de
dc.creatorDias, Fátima Ribeiro
dc.date.accessioned2025-03-24T12:46:42Z
dc.date.available2025-03-24T12:46:42Z
dc.date.issued2017
dc.description.abstractPlatelet-activating factor (PAF) is produced by macrophages during inflammation and infections. We evaluated whether PAF is able to modulate the infection of human macrophages by Leishmania braziliensis, the main Leishmania sp. in Brazil. Monocyte-derived macrophages were incubated with promastigote forms in absence or presence of exogenous PAF. We observed that the treatment of macrophages with low concentrations of PAF prior to infection increased the phagocytosis of L. braziliensis. More importantly, exogenous PAF reduced the parasitism when it was added before, during or after infection. In addition, treatment with a PAF antagonist (PCA 4248) resulted in a significant increase of macrophage infection in a concentration-dependent manner, suggesting that endogenous PAF is important to control L. braziliensis infection. Mechanistically, while exogenous PAF increased production of reactive oxygen species (ROS) treatment with PCA 4248 reduced oxidative burst during L. braziliensis infection. The microbicidal effects of exogenous PAF were abolished when macrophages were treated with apocynin, an NADPH oxidase inhibitor. The data show that PAF promotes the production of ROS induced by L. braziliensis, suggesting that this lipid mediator may be relevant to control L. braziliensis infection in human macrophages.
dc.identifier.citationBORGES, Arissa Felipe et al. Platelet-activating factor increases reactive oxygen species-mediated microbicidal activity of human macrophages infected with Leishmania (Viannia) braziliensis. Pathogens and Disease, Oxford, v. 75, n. 7, eftx082, 2017. DOI: 10.1093/femspd/ftx082. Disponível em: https://academic.oup.com/femspd/article/75/7/ftx082/3983173?login=true. Acesso em: 20 mar. 2025.
dc.identifier.doi10.1093/femspd/ftx082
dc.identifier.issne- 2049-632X
dc.identifier.urihttp://repositorio.bc.ufg.br//handle/ri/27063
dc.language.isoeng
dc.publisher.countryGra-bretanha
dc.publisher.departmentInstituto de Patologia Tropical e Saúde Pública - IPTSP (RMG)
dc.rightsAcesso Aberto
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectLeishmania (Viannia) braziliensis
dc.subjectHuman macrophage
dc.subjectPlatelet-activating factor
dc.subjectReactive oxygen species
dc.titlePlatelet-activating factor increases reactive oxygen species-mediated microbicidal activity of human macrophages infected with Leishmania (Viannia) braziliensis
dc.typeArtigo

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