Transcriptional profiles of the human pathogenic fungus paracoccidioides brasiliensis in mycelium and yeast cells
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2005-07
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Paracoccidioides brasiliensis is the causative agent of
paracoccidioidomycosis, a disease that affects 10 million
individuals in Latin America. This report depicts the
results of the analysis of 6,022 assembled groups from
mycelium and yeast phase expressed sequence tags, cov-
ering about 80% of the estimated genome of this dimor-
phic, thermo-regulated fungus. The data provide a com-
prehensive view of the fungal metabolism, including
overexpressed transcripts, stage-specific genes, and
also those that are up- or down-regulated as assessed by
in silico electronic subtraction and cDNA microarrays.
Also, a significant differential expression pattern in my-
celium and yeast cells was detected, which was con-
firmed by Northern blot analysis, providing insights
into differential metabolic adaptations. The overall
transcriptome analysis provided information about se-
quences related to the cell cycle, stress response, drug
resistance, and signal transduction pathways of the pathogen. Novel P. brasiliensis genes have been identi-
fied, probably corresponding to proteins that should be
addressed as virulence factor candidates and potential
new drug targets.
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FELIPE , Maria Sueli S. et al. Transcriptional profiles of the human pathogenic fungus paracoccidioides brasiliensis in mycelium and yeast cells. Journal of Biological Chemistry, Rockville, v. 280, n. 26, p. 24707-24714, July 2005.