A new approach for the analysis of amino acid neurotransmitters in mouse brain tissues using DESI imaging

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dc.creatorMaciel, Lanaia Ítala Louzeiro
dc.creatorSilva, Igor Pereira da
dc.creatorRamalho, Ruver Rodrigues Feitosa
dc.creatorPinto, Mauro Cunha Xavier
dc.creatorVaz, Boniek Gontijo
dc.date.accessioned2023-07-12T12:43:05Z
dc.date.available2023-07-12T12:43:05Z
dc.date.issued2022
dc.description.abstractMass spectrometry imaging using desorption electrospray ionization (DESI imaging) is a method with the potential to investigate the distribution of neurotransmitters (biogenic amines and amino acids) in brain tissues. Because of the low ionization efficiency of neurotransmitters in direct tissue analysis by DESI, neurotransmitters can be analyzed using derivatization protocols that insert a known compound into their structures through a chemical reaction due to signal suppression by lipids. In this sense, this work aims to combine a chemical derivatization protocol with DESI imaging in order to improve the analysis sensitivity of the amino acids glycine (Gly), alanine (Ala), dopamine (Dop), and gamma-aminobutyric acid (GABA) in mouse brain tissues, as well as to apply this protocol to analyze mice with Parkinson's disease. Derivatization took place using the reagent 2,4,6-triphenylpyrylium tetrafluoroborate (TPP+) and was first investigated in a solvent to ensure the detection of the expected products. Subsequently, the brain tissue surface was subjected to the derivatization solution through spraying. Then, the tissue was analyzed by DESI, and the derivatized Gly, Ala, Dop, and GABA were detected. DESI imaging analysis demonstrated distinct spatial distributions for these derivatized amino acid neurotransmitters. The application of the protocol in brain sections from mice with Parkinson's disease demonstrated differences in the intensities and anatomical distribution for the derivatized amino acids compared with control tissues (unaffected by Parkinson's). The protocol combining derivatization with DESI imaging described in this work emerges as a potential tool for analyzing Parkinson's disease biomarkers in mouse brain tissues.pt_BR
dc.identifier.citationMACIEL, Lanaia I. L. et al. A new approach for the analysis of amino acid neurotransmitters in mouse brain tissues using DESI imaging. International Journal of Mass Spectrometry, Amsterdam, v. 471, e116730, 2022. DOI: 10.1016/j.ijms.2021.116730. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S1387380621002116?via%3Dihub. Acesso em: 28 jun. 2023.pt_BR
dc.identifier.doi10.1016/j.ijms.2021.116730
dc.identifier.issn1387-3806
dc.identifier.issne- 1873-2798
dc.identifier.urihttps://www.sciencedirect.com/science/article/abs/pii/S1387380621002116?via%3Dihub
dc.language.isoengpt_BR
dc.publisher.countryHolandapt_BR
dc.publisher.departmentInstituto de Química - IQ (RMG)pt_BR
dc.rightsAcesso Restritopt_BR
dc.titleA new approach for the analysis of amino acid neurotransmitters in mouse brain tissues using DESI imagingpt_BR
dc.typeArtigopt_BR

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