Application of polymeric nanoparticles for controlled release of ethanolic extract of guapeva leaves (Pouteria gardneriana Radlk) against Riphicephalus (Boophilus) microplus through in vitro studies
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2016-12
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The study was designed to evaluate the ethanolic extract acaricide action from Pouteria gardneriana
Radlk specie adsorbed in chitosan nanoparticles for controlled release against Riphicephalus
(Boophilus) microplus. Leaves of P. gardneriana Radlk were collected, air-dried, ground and extracted
with ethanol. Chitosan nanospheres were obtained by the phase inversion method. Conductometric
titration, UV-Vis and FTIR analysis were conducted with the materials to evaluate the chitosan
anchoring ability. In vitro test was performed using engorged females for each treatment, which
consisted of the control, raw extract and three treatments diluted, with increasing concentrations of 0.2,
0.4 and 1%. The most effectiveness extract was for 0.4% concentration by observing reduction in the
eggs mass compared to control group. The nanomaterial proved to be able to anchor and release the
insecticide gradually in pH between 5 and 6 regions, which makes it feasible for use in cattle,
prolonging the exposure time between the tick and acaricide.
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Extracts, Nanoparticle, Controlled release, Tick
Citação
BARBOSA, Priscila Fernanda Pereira et al. Application of polymeric nanoparticles for controlled release of ethanolic extract of guapeva leaves (Pouteria gardneriana Radlk) against Riphicephalus (Boophilus) microplus through in vitro studies. African Journal of Biotechnology, Lagos, v. 15, n. 49, p. 2778-2786, Dec. 2016. DOI: 10.5897/AJB2016.15230. Disponível em: https://academicjournals.org/journal/AJB/article-abstract/60AFE0A61937. Acesso em: 17 maio 2023.