Aproveitamento da coroa do abacaxi para obtenção de açúcares redutores via hidrólise enzimática com uso de bromelina extraída in situ

Abstract

The pineapple crown is a lignocellulosic biomass with the potential to obtain products with greater added value. This study proposes an integrated approach for the use of this waste, involving the protection of the proteolytic enzyme bromelain, widely applied in several industries, and the obtaining of reducing sugars with potential for fermentation into bioproducts. An analysis of the behavior of protein concentration during the nutrition stage revealed that ideal conditions were achieved in phosphate supply with pH 8.06 and an L/S ratio of 1:1, resulting in 1.2619 g/L of proteins total or 2.52% (m/m) of bromelain, a higher result than that found in previous studies. In the subsequent enzymatic hydrolysis step, the use of bromelain as an additive provided a significant increase in glucose and reducing sugars, since concentrations of 8.08 g/L and 17.49 g/L were obtained without the use of bromelain and 17, 21 g/L and 26.14 g/L using this protein.